Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,100,922	C→T	L234L (CTG→CTA)	yggN ←	putative EcfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,100,922	0	C	T	100.0%	67.6 / NA	20	L234L (CTG→CTA)	yggN	putative EcfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/11);  total (9/11)

AAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTC  >  NC_000913/3100856‑3100996                                                                   |                                                                           aagaaTTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATACAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCt                                                              >  1:539211/1‑81 (MQ=255)    aaTTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATc                                                           <  2:539192/81‑1 (MQ=255)       tAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTc                                                        >  2:539221/1‑81 (MQ=255)               aTAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTca                                                >  1:539214/1‑81 (MQ=255)                        tattatGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGc                                       <  1:539193/81‑1 (MQ=255)                          ttatGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTa                                     >  1:539213/1‑81 (MQ=255)                            atGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTAc                                    <  1:539194/80‑1 (MQ=255)                              gCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACaaa                                 >  2:539216/1‑81 (MQ=255)                               ccGTCTTAAAATAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAAc                                <  1:539195/81‑1 (MQ=255)                                      aaaaTAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCttt                          <  2:539198/80‑1 (MQ=255)                                      aaaaTAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCtt                           <  1:539196/79‑1 (MQ=255)                                      aaaaTAGAGGAATATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCtt                           <  1:539197/79‑1 (MQ=255)                                          tAGAGGATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCaa                     <  1:539199/81‑1 (MQ=255)                                              ggATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAActg                 <  2:539200/81‑1 (MQ=255)                                               gATTATTTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAActgc                >  1:539217/1‑81 (MQ=255)                                                attattTTAAATTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAActgct               <  1:539201/81‑1 (MQ=255)                                                         aaTTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCtt      >  2:539225/1‑81 (MQ=255)                                                         aaTTCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCt       <  2:539202/80‑1 (MQ=255)                                                            tCCCGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCttttt   >  2:539229/1‑81 (MQ=255)                                                             cccGACTAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTc  >  1:539222/1‑81 (MQ=255)                                                                   |                                                                           AAGAATTAAACGGCATAAAAAAGTATTATGCCGTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTC  >  NC_000913/3100856‑3100996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑