Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,149,763	G→T	P34P (CCG→CCT)	yghA →	NADP(+)‑dependent aldehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,149,763	0	G	T	100.0%	95.0 / NA	28	P34P (CCG→CCT)	yghA	NADP(+)‑dependent aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/14);  total (14/14)

ACGCAGTATTACACTGGTGAATATCCCAAACAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCGGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGACAGGGGGCGATTCC  >  NC_000913/3149686‑3149838                                                                              |                                                                            aCGCAGTATTACACTGGTGAATATCCCAAACAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGAt                                                                          >  1:549223/1‑81 (MQ=255)    cAGTATTACACTGGTGAATATCCCAAACAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTgc                                                                       <  2:549187/81‑1 (MQ=255)           acacTGGTGAATATCCCAAACAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCgag                                                                 >  1:549229/1‑80 (MQ=255)           acacTGGTGAATATCCCAAACAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCgag                                                                 >  1:549226/1‑80 (MQ=255)                    aaTATCCCAAACAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTAt                                                        <  2:549188/80‑1 (MQ=255)                          ccAAACAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTa                                                 <  1:549189/81‑1 (MQ=255)                            aaaCAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGc                                               >  2:549219/1‑81 (MQ=255)                            aaaCAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGc                                               <  2:549190/81‑1 (MQ=255)                              aCAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCgg                                             <  1:549191/81‑1 (MQ=255)                                aGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTc                                           <  1:549192/81‑1 (MQ=255)                                aGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTc                                           <  1:549193/81‑1 (MQ=255)                                    acaacCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCt                                       <  2:549194/81‑1 (MQ=255)                                     caacCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTg                                      <  1:549195/81‑1 (MQ=255)                                       acCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGa                                     >  1:549221/1‑80 (MQ=255)                                         cGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAg                                  <  2:549196/81‑1 (MQ=255)                                             gCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGa                                     >  2:549197/1‑74 (MQ=255)                                             gCCAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCg                              >  2:549218/1‑81 (MQ=255)                                               cAGGCATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGt                             >  2:549220/1‑80 (MQ=255)                                                   cATCCAGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGc                        <  2:549198/81‑1 (MQ=255)                                                        aGGCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTgg                   <  1:549199/81‑1 (MQ=255)                                                          gCGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTg                 <  2:549200/81‑1 (MQ=255)                                                           cGAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGa                >  1:549231/1‑81 (MQ=255)                                                            gAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGa                >  2:549235/1‑80 (MQ=255)                                                            gAAGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGAc               <  2:549201/81‑1 (MQ=255)                                                             aaGATGACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGa                >  2:549243/1‑79 (MQ=255)                                                                  gACACCGGTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGACAggggg         >  1:549225/1‑81 (MQ=255)                                                                        ggTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGACAGGGGGCGATTc   >  2:549222/1‑81 (MQ=255)                                                                         gTACCTGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGACAGGGGGCGATTcc  >  1:549237/1‑81 (MQ=255)                                                                              |                                                                            ACGCAGTATTACACTGGTGAATATCCCAAACAGAAACAACCGACGCCAGGCATCCAGGCGAAGATGACACCGGTACCGGATTGCGGCGAGAAAACCTATGTTGGTAGCGGTCGCCTGAAAGATCGTAAAGCACTGGTGACAGGGGGCGATTCC  >  NC_000913/3149686‑3149838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑