Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,155,049	G→A	T57M (ACG→ATG)	yqhC ←	DNA‑binding transcriptional activator YqhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,155,049	0	G	A	100.0%	110.7 / NA	32	T57M (ACG→ATG)	yqhC	DNA‑binding transcriptional activator YqhC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/21);  total (11/21)

ATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATT  >  NC_000913/3154974‑3155119                                                                            |                                                                       atatAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCg                                                                   <  2:550187/81‑1 (MQ=255)    taACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGcc                                                                 <  1:550188/80‑1 (MQ=255)             ttATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACaaa                                                       >  1:550224/1‑81 (MQ=255)                tGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGc                                                    <  1:550189/81‑1 (MQ=255)                 gCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGca                                                   >  2:550226/1‑81 (MQ=255)                    cGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACAt                                                <  1:550190/81‑1 (MQ=255)                     ggAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATc                                               >  2:550230/1‑81 (MQ=255)                     ggAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATc                                               <  1:550191/81‑1 (MQ=255)                      gAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCg                                              <  2:550192/81‑1 (MQ=255)                          agagAATTATGATGCCAGGCTCATACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGc                                           >  1:550250/1‑80 (MQ=255)                             gaATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCaa                                         >  2:550232/1‑79 (MQ=255)                              aaTTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAg                                      <  2:550194/81‑1 (MQ=255)                              aaTTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAg                                      <  1:550193/81‑1 (MQ=255)                                 tATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTcc                                   >  1:550222/1‑81 (MQ=255)                                   tgatgCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCt                                  <  2:550195/80‑1 (MQ=255)                                   tgatgCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTg                                 <  2:550196/81‑1 (MQ=255)                                     atgCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGa                                >  1:550223/1‑80 (MQ=255)                                            gCTCGTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAt                        <  1:550197/81‑1 (MQ=255)                                                gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAttt                      <  1:550200/79‑1 (MQ=255)                                                gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAttt                      <  1:550199/79‑1 (MQ=255)                                                gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTc                    >  1:550235/1‑81 (MQ=255)                                                gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTc                    <  2:550201/81‑1 (MQ=255)                                                gTACATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTc                    <  1:550198/81‑1 (MQ=255)                                                  aCATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCtt                  >  2:550234/1‑81 (MQ=255)                                                   cATCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCtt                  >  1:550228/1‑80 (MQ=255)                                                    aTCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTa                <  1:550202/81‑1 (MQ=255)                                                     tCACCGGTGTACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTAt               <  2:550203/81‑1 (MQ=255)                                                            tgtACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAg        <  1:550204/81‑1 (MQ=255)                                                              tACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGtt      <  1:550206/81‑1 (MQ=255)                                                              tACGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGtt      <  1:550205/81‑1 (MQ=255)                                                                cGTGCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGttt     <  2:550207/80‑1 (MQ=255)                                                                   gCGAAAGGCATCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTttatt  >  2:550231/1‑80 (MQ=255)                                                                            |                                                                       ATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATT  >  NC_000913/3154974‑3155119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑