Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,156,186	G→A	G278S (GGT→AGT)	yqhD →	NADPH‑dependent aldehyde reductase YqhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,156,186	0	G	A	100.0%	181.2 / NA	51	G278S (GGT→AGT)	yqhD	NADPH‑dependent aldehyde reductase YqhD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

ACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCTAA  >  NC_000913/3156112‑3156262                                                                           |                                                                             aCGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTg                                                                         >  2:550474/1‑80 (MQ=255)   ggTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGAt                                                                      >  1:550481/1‑81 (MQ=255)   ggTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGAt                                                                      >  1:550467/1‑81 (MQ=255)        gATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCAcg                                                                  <  1:550406/80‑1 (MQ=255)         aTTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCAcgcg                                                                >  1:550513/1‑81 (MQ=255)         aTTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCAcgcg                                                                >  1:550466/1‑81 (MQ=255)         aTTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCAcgc                                                                 <  2:550407/80‑1 (MQ=255)               gcTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAaca                                                          >  2:550477/1‑81 (MQ=255)               gcTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAaca                                                          >  1:550458/1‑81 (MQ=255)                 tGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACt                                                        >  2:550464/1‑81 (MQ=255)                   gCGTACCGCAGGACTGGGCAACGCATATGCTGGGCGACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTg                                                       <  1:550408/80‑1 (MQ=255)                    cGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGc                                                     >  1:550512/1‑81 (MQ=255)                        ccGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTAt                                                  <  1:550409/80‑1 (MQ=255)                        ccGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATc                                                 >  2:550484/1‑81 (MQ=255)                        ccGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATc                                                 <  1:550410/81‑1 (MQ=255)                         cGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCg                                                >  2:550462/1‑81 (MQ=255)                         cGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCg                                                >  2:550473/1‑81 (MQ=255)                            aGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcc                                             >  1:550465/1‑81 (MQ=255)                            aGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcc                                             >  1:550471/1‑81 (MQ=255)                              gACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctg                                           >  1:550479/1‑81 (MQ=255)                               aCTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctgc                                          >  1:550470/1‑81 (MQ=255)                               aCTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctgc                                          <  1:550411/81‑1 (MQ=255)                                  gggCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctgcctg                                       >  2:550483/1‑81 (MQ=255)                                  gggCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTcctgcctg                                       >  2:550515/1‑81 (MQ=255)                                     cAACGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCAc                                    >  2:550501/1‑81 (MQ=255)                                      aaCGCATATGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACt                                   <  2:550412/81‑1 (MQ=255)                                           atatGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTgg                               >  2:550475/1‑80 (MQ=255)                                           atatGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                              <  1:550413/81‑1 (MQ=255)                                           atatGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                              <  2:550414/81‑1 (MQ=255)                                           atatGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                              <  1:550415/81‑1 (MQ=255)                                              tGCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATg                           <  1:550416/81‑1 (MQ=255)                                               gCTGGGCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATg                           >  1:550478/1‑80 (MQ=255)                                                  gggCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGaaa                        >  2:550493/1‑80 (MQ=255)                                                   ggCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGaaaa                       <  2:550417/80‑1 (MQ=255)                                                    gCCACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAAc                     <  1:550418/81‑1 (MQ=255)                                                     ccACGAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAAcg                    <  1:550419/81‑1 (MQ=255)                                                         gAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGa                 >  2:550496/1‑80 (MQ=255)                                                         gAACTGACTGCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGa                 >  2:550488/1‑80 (MQ=255)                                                           actgactgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATAc              <  1:550420/81‑1 (MQ=255)                                                            ctgactgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATAcc             <  2:550421/81‑1 (MQ=255)                                                               actgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATGCCa            <  2:550425/79‑1 (MQ=255)                                                               actgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCa            <  1:550423/79‑1 (MQ=255)                                                               actgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCa            <  2:550422/79‑1 (MQ=255)                                                               actgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAg          <  2:550424/81‑1 (MQ=255)                                                                ctgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAgc         >  1:550486/1‑81 (MQ=255)                                                                ctgCGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAgc         <  2:550426/81‑1 (MQ=255)                                                                   cGATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAgcgc       <  2:550427/80‑1 (MQ=255)                                                                    gATGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAgcgcgc     >  2:550476/1‑81 (MQ=255)                                                                     aTGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCt    <  2:550430/81‑1 (MQ=255)                                                                     aTGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCt    <  2:550429/81‑1 (MQ=255)                                                                     aTGCACAGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCt    <  2:550428/81‑1 (MQ=255)                                                                        cacaGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCTaa  <  1:550431/80‑1 (MQ=255)                                                                           |                                                                             ACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGATACCAAGCGCGCTAA  >  NC_000913/3156112‑3156262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑