Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,695	C→T	S224S (AGC→AGT)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,695	0	C	T	100.0%	142.4 / NA	41	S224S (AGC→AGT)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/23);  total (18/23)

GTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGG  >  NC_000913/3208618‑3208771                                                                              |                                                                             gttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCa                                                                           >  1:560638/1‑81 (MQ=255)  ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCAt                                                                          <  1:560589/81‑1 (MQ=255)  ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCAt                                                                          >  1:560637/1‑81 (MQ=255)     ccGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCAtctc                                                                       <  1:560590/81‑1 (MQ=255)       gACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTcac                                                                     <  1:560591/81‑1 (MQ=255)            cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTcacgcc                                                                  <  2:560593/79‑1 (MQ=255)            cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTcacgcc                                                                  <  1:560592/79‑1 (MQ=255)             gtcaCCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTcacgccacg                                                               <  2:560594/78‑1 (MQ=255)                       ccATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTggg                                                     >  1:560648/1‑81 (MQ=255)                        cATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTgggg                                                    >  1:560642/1‑81 (MQ=255)                         aTTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGc                                                   >  1:560643/1‑81 (MQ=255)                          ttttCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCg                                                  <  1:560595/81‑1 (MQ=255)                              cTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTg                                              >  1:560661/1‑81 (MQ=255)                               ttGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTgg                                             >  1:560669/1‑81 (MQ=255)                                tGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGt                                            >  1:560653/1‑81 (MQ=255)                                 gACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTg                                              <  2:560596/78‑1 (MQ=255)                                 gACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGtt                                           >  2:560644/1‑81 (MQ=255)                                   cGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTcc                                         <  2:560597/81‑1 (MQ=255)                                    ggCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCt                                        <  2:560598/81‑1 (MQ=255)                                     gCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGGATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTc                                       <  2:560599/81‑1 (MQ=255)                                       gATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCgg                                     <  2:560600/81‑1 (MQ=255)                                        aTGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCggg                                    <  2:560601/81‑1 (MQ=255)                                           gCACCAAACTTGCTGTTAATTGGACTGATGAGAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATg                                 <  2:560602/81‑1 (MQ=255)                                           gCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGAt                                  <  1:560603/80‑1 (MQ=255)                                           gCACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATg                                 >  2:560654/1‑81 (MQ=255)                                            cACCAAACTTGCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGt                                >  2:560652/1‑81 (MQ=255)                                                      gCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTc                        <  2:560604/79‑1 (MQ=255)                                                      gCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTc                        <  1:560605/79‑1 (MQ=255)                                                      gCTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAg                      >  2:560667/1‑81 (MQ=255)                                                       cTGTTAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAg                      <  2:560606/80‑1 (MQ=255)                                                          ttAATTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCAtt                  <  2:560607/81‑1 (MQ=255)                                                            aaTTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCAttt                 <  1:560608/80‑1 (MQ=255)                                                             aTTGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCAtttt                <  2:560609/80‑1 (MQ=255)                                                              ttGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTAc              >  1:560671/1‑81 (MQ=255)                                                               tGGACTGATGAAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACt             >  1:560646/1‑81 (MQ=255)                                                                    tgatgaAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTc        >  1:560679/1‑81 (MQ=255)                                                                    tgatgaAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTc        >  2:560659/1‑81 (MQ=255)                                                                      atgaAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTc        <  2:560610/79‑1 (MQ=255)                                                                        gaAAAGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgct    >  1:560655/1‑81 (MQ=255)                                                                          aaaGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTgg  <  2:560611/81‑1 (MQ=255)                                                                          aaaGTGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTgg  >  2:560672/1‑81 (MQ=255)                                                                              |                                                                             GTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGG  >  NC_000913/3208618‑3208771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑