Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,918	C→T	P96S (CCG→TCG)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,222,918	0	C	T	100.0%	91.4 / NA	27	P96S (CCG→TCG)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/17);  total (10/17)

ACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGAC  >  NC_000913/3222841‑3222995                                                                              |                                                                              aCTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGt                                                                            <  1:563644/81‑1 (MQ=255)  cTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGtt                                                                           >  2:563678/1‑81 (MQ=255)    gggggCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTcc                                                                         >  1:563690/1‑81 (MQ=255)       ggCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCa                                                                      <  1:563646/81‑1 (MQ=255)        gCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCAt                                                                     <  1:563647/81‑1 (MQ=255)        gCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCAt                                                                     >  1:563683/1‑81 (MQ=255)         cATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATc                                                                    <  2:563649/81‑1 (MQ=255)         cATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATc                                                                    <  2:563651/81‑1 (MQ=255)         cATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATc                                                                    <  2:563650/81‑1 (MQ=255)         cATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATc                                                                    <  1:563648/81‑1 (MQ=255)                   tCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGt                                                          <  2:563652/81‑1 (MQ=255)                     cccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGtt                                                         <  2:563653/80‑1 (MQ=255)                       cGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGt                                                      >  1:563686/1‑81 (MQ=255)                            tgtgGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCa                                                 <  2:563654/81‑1 (MQ=255)                                gCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGa                                             >  2:563697/1‑81 (MQ=255)                                 cAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGAt                                            <  1:563655/81‑1 (MQ=255)                                          ggTCACGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAAcc                                   >  1:563692/1‑81 (MQ=255)                                               cGGCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGc                              <  1:563656/81‑1 (MQ=255)                                                ggCAAACTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGcc                             >  1:563681/1‑81 (MQ=255)                                                     aCTGCAATATACCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCa                        <  2:563657/81‑1 (MQ=255)                                                             ataCCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTAttt                >  1:563685/1‑81 (MQ=255)                                                              taCCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTAttt                <  2:563658/80‑1 (MQ=255)                                                               aCCGACGAAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTAtttt               >  2:563696/1‑80 (MQ=255)                                                                     gaAGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTc        <  2:563659/81‑1 (MQ=255)                                                                      aaGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCt         >  1:563693/1‑79 (MQ=255)                                                                       aGGTTTTTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAg      <  2:563660/81‑1 (MQ=255)                                                                          ttttTCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGa   <  1:563661/81‑1 (MQ=255)                                                                           ttttCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGAc  >  2:563694/1‑81 (MQ=255)                                                                              |                                                                              ACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGAC  >  NC_000913/3222841‑3222995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑