Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,136	C→T	A249V (GCC→GTC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,136	0	C	T	100.0%	141.1 / NA	40	A249V (GCC→GTC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/16);  total (24/16)

AAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCG  >  NC_000913/23058‑23212                                                                               |                                                                             aaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCa                                                                            <  1:4226/81‑1 (MQ=255) aaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCa                                                                            <  2:4227/81‑1 (MQ=255)  aGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCaa                                                                           >  2:4284/1‑81 (MQ=255)    cAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAAcc                                                                         >  2:4299/1‑81 (MQ=255)     aaaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACcg                                                                        >  1:4293/1‑81 (MQ=255)      aaaTTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACcgc                                                                       >  2:4289/1‑81 (MQ=255)            gCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAAtc                                                                 >  2:4287/1‑81 (MQ=255)               gTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAAtctc                                                               >  2:4274/1‑80 (MQ=255)                tAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTa                                                             <  1:4228/81‑1 (MQ=255)                tAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTa                                                             >  1:4315/1‑81 (MQ=255)                 aaGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTAt                                                            >  2:4281/1‑81 (MQ=255)                  aGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTAtt                                                           >  1:4303/1‑81 (MQ=255)                   gCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTg                                                          <  2:4229/81‑1 (MQ=255)                   gCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTg                                                          <  1:4230/81‑1 (MQ=255)                    cAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGc                                                         <  1:4231/81‑1 (MQ=255)                      aCGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCAc                                                       <  2:4232/81‑1 (MQ=255)                        gTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCAc                                                       >  1:4297/1‑79 (MQ=255)                        gTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCAc                                                       >  1:4317/1‑79 (MQ=255)                          tAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCAc                                                       >  2:4285/1‑77 (MQ=255)                           aaCGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGAt                                                  >  1:4279/1‑81 (MQ=255)                            aCGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGAtt                                                 >  1:4254/1‑81 (MQ=255)                             cGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGAttt                                                >  2:4305/1‑81 (MQ=255)                              ggCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTc                                               <  2:4234/81‑1 (MQ=255)                              ggCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTc                                               <  1:4233/81‑1 (MQ=255)                                   aaTCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTa                                          >  2:4292/1‑81 (MQ=255)                                    aTCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTAt                                         >  1:4275/1‑81 (MQ=255)                                    aTCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTAt                                         <  1:4235/81‑1 (MQ=255)                                            ggTAATCTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGt                                 >  1:4300/1‑81 (MQ=255)                                                aTCTGGACCACCACGCCGTAGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCa                              <  1:4236/80‑1 (MQ=255)                                                  cTGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTg                                >  2:4288/1‑76 (MQ=255)                                                   tGGACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTg                                >  1:4298/1‑75 (MQ=255)                                                     gACCACCACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATc                          <  2:4237/79‑1 (MQ=255)                                                      accaccACGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGAc                       <  2:4238/81‑1 (MQ=255)                                                            acGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAg                 <  2:4240/81‑1 (MQ=255)                                                            acGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAg                 >  2:4291/1‑81 (MQ=255)                                                            acGCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAg                 <  2:4239/81‑1 (MQ=255)                                                              gCCGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGc               >  1:4311/1‑81 (MQ=255)                                                               ccGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGcc              <  2:4241/81‑1 (MQ=255)                                                                cGTGGACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCg             >  1:4294/1‑81 (MQ=255)                                                                   ggACTCTGCCTGTCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGa          >  2:4313/1‑81 (MQ=255)                                                                          gcctgtcAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGc   >  1:4290/1‑81 (MQ=255)                                                                          gcctgtcAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGc   >  1:4301/1‑81 (MQ=255)                                                                          gcctgtcAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGc   >  2:4310/1‑81 (MQ=255)                                                                            ctgtcAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCg  >  2:4324/1‑80 (MQ=255)                                                                               |                                                                             AAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCG  >  NC_000913/23058‑23212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑