Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,225,566	C→T	S978S (AGC→AGT)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,225,566	0	C	T	100.0%	104.2 / NA	31	S978S (AGC→AGT)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/18);  total (13/18)

CTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGG  >  NC_000913/3225492‑3225640                                                                           |                                                                           cTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTg                                                                      <  1:564271/81‑1 (MQ=255) cTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTg                                                                      >  2:564330/1‑81 (MQ=255)    cAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCt                                                                    <  2:564272/80‑1 (MQ=255)     aGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTgg                                                                  <  1:564273/81‑1 (MQ=255)     aGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTgg                                                                  <  2:564274/81‑1 (MQ=255)         cAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTc                                                              >  1:564312/1‑81 (MQ=255)           gTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCt                                                            >  2:564313/1‑81 (MQ=255)            tgatgaCATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTg                                                           <  1:564275/81‑1 (MQ=255)            tgatgaCATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTg                                                           <  2:564276/81‑1 (MQ=255)                  cATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGt                                                     >  1:564329/1‑81 (MQ=255)                    tCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCt                                                   <  2:564277/81‑1 (MQ=255)                    tCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCt                                                   <  1:564278/81‑1 (MQ=255)                     cACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTg                                                  >  2:564323/1‑81 (MQ=255)                      aCCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTgg                                                 >  2:564320/1‑81 (MQ=255)                                  gATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTc                                     <  2:564280/81‑1 (MQ=255)                                  gATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTc                                     <  1:564279/81‑1 (MQ=255)                                        cAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTAc                               <  1:564282/81‑1 (MQ=255)                                        cAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTAc                               >  1:564342/1‑81 (MQ=255)                                        cAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTAc                               <  1:564281/81‑1 (MQ=255)                                         aGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACg                              >  2:564343/1‑81 (MQ=255)                                              ctTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCttt                         <  1:564283/81‑1 (MQ=255)                                              ctTGGCCTCGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCt                           <  1:564284/79‑1 (MQ=255)                                                      cGGCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgct                 >  2:564333/1‑81 (MQ=255)                                                       ggCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgctg                <  2:564285/81‑1 (MQ=255)                                                       ggCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgctg                <  2:564286/81‑1 (MQ=255)                                                        gCTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTtgctgc               >  2:564336/1‑81 (MQ=255)                                                         cTCCAACTCCTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGcc              <  2:564287/81‑1 (MQ=255)                                                                  cTGGGGCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGAGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTgg     >  1:564332/1‑81 (MQ=255)                                                                    ggggCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCg   >  1:564331/1‑81 (MQ=255)                                                                    ggggCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCg   >  2:564341/1‑81 (MQ=255)                                                                     gggCAGTGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCgg  <  1:564288/81‑1 (MQ=255)                                                                           |                                                                           CTGCAGCGCAGTGATGACATCACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTGCTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTTTCTGGCGG  >  NC_000913/3225492‑3225640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑