Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,259,347	A→G	G101G (GGT→GGC)	tdcG ←	L‑serine deaminase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,259,347	0	A	G	100.0%	82.6 / NA	24	G101G (GGT→GGC)	tdcG	L‑serine deaminase III
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/9);  total (15/9)

CATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCT  >  NC_000913/3259269‑3259423                                                                               |                                                                             cATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGcc                                                                            <  1:571054/81‑1 (MQ=255)     ccGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGAt                                                                        >  2:571098/1‑81 (MQ=255)      cGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTCCTACAGGAAAATCAACAATATGCGCGCCAGATg                                                                       <  2:571055/81‑1 (MQ=255)             aTGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTgg                                                                <  1:571056/81‑1 (MQ=255)             aTGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTgg                                                                >  1:571078/1‑81 (MQ=255)               ggCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTgg                                                                >  1:571082/1‑79 (MQ=255)                  gAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAgcc                                                           >  1:571090/1‑81 (MQ=255)                  gAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAgcc                                                           <  2:571057/81‑1 (MQ=255)                              cGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTgcgc                                                <  1:571058/80‑1 (MQ=255)                              cGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGcgcg                                               >  2:571093/1‑81 (MQ=255)                               gggATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGcgcg                                               <  2:571059/80‑1 (MQ=255)                               gggATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGt                                              <  1:571060/81‑1 (MQ=255)                                 gATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTa                                            >  1:571081/1‑81 (MQ=255)                                         aTAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAActc                                     >  1:571097/1‑80 (MQ=255)                                           aaTGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCta                                   >  1:571102/1‑80 (MQ=255)                                           aaTGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCta                                   >  1:571094/1‑80 (MQ=255)                                             tGTTCTTTGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCtata                                 >  1:571095/1‑80 (MQ=255)                                                  tttGCTACAGGAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATg                             >  1:571083/1‑79 (MQ=255)                                                           ggAAAATCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCtca                  >  1:571099/1‑81 (MQ=255)                                                             aaaaTCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCtcatc                <  1:571061/81‑1 (MQ=255)                                                             aaaaTCAACAATATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCtcat                 >  2:571106/1‑80 (MQ=255)                                                                  caacaaTATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGa           >  1:571109/1‑81 (MQ=255)                                                                   aacaaTATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGa           >  2:571105/1‑80 (MQ=255)                                                                      aaTATGCGCGCCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGacaac       <  2:571062/81‑1 (MQ=255)                                                                           gcgcgcCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCt  >  2:571100/1‑81 (MQ=255)                                                                               |                                                                             CATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGCGCACCAGATGCCACTGGCAGCCGCCCGCTGCGCGTTACTAACTCTATAAATGCAGGGATCTCATCAATGACAACATCCT  >  NC_000913/3259269‑3259423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑