Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,297,253	C→T	A296T (GCT→ACT)	yraQ ←	permease family protein YraQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,297,253	0	C	T	100.0%	141.4 / NA	40	A296T (GCT→ACT)	yraQ	permease family protein YraQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/22);  total (18/22)

GGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTG  >  NC_000913/3297178‑3297330                                                                            |                                                                              ggAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTc                                                                          <  2:578028/81‑1 (MQ=255) ggAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTc                                                                          >  1:578073/1‑81 (MQ=255)   aaCGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTccc                                                                        <  1:578029/81‑1 (MQ=255)    aCGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCa                                                                       >  2:578068/1‑81 (MQ=255)       cTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATAc                                                                    >  1:578079/1‑81 (MQ=255)        tttGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATAcc                                                                   >  1:578075/1‑81 (MQ=255)            cgcAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTTGTTCCCATACCTGcc                                                               <  2:578030/81‑1 (MQ=255)            cgcAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGc                                                                >  2:578096/1‑80 (MQ=255)             gcagcaTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGc                                                                >  2:578074/1‑79 (MQ=255)              cagcaTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAg                                                             >  2:578078/1‑81 (MQ=255)               agcaTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGc                                                            <  2:578031/81‑1 (MQ=255)                 caTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGcat                                                          <  2:578032/81‑1 (MQ=255)                  aTAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGcat                                                          >  1:578071/1‑80 (MQ=255)                    aaTCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGcatcat                                                       >  2:578087/1‑81 (MQ=255)                     aTCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGcatcat                                                       <  2:578035/80‑1 (MQ=255)                     aTCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCatcatc                                                      <  2:578034/81‑1 (MQ=255)                     aTCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCatcatc                                                      <  2:578033/81‑1 (MQ=255)                      tCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCg                                                     <  2:578036/81‑1 (MQ=255)                        aGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGtt                                                   <  2:578037/81‑1 (MQ=255)                         gTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGttt                                                  >  1:578084/1‑81 (MQ=255)                           gACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTg                                                 >  1:578080/1‑80 (MQ=255)                            aCGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTa                                               <  2:578038/81‑1 (MQ=255)                                   aCTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCgg                                        <  2:578039/81‑1 (MQ=255)                                          gCCGGGAGCGTCATTAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTct                                 >  2:578086/1‑81 (MQ=255)                                             gggAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgct                              <  1:578040/81‑1 (MQ=255)                                              ggggCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgctg                             <  1:578042/78‑1 (MQ=255)                                              ggAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgctg                             >  2:578066/1‑81 (MQ=255)                                              ggAGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTctgctg                             <  1:578041/81‑1 (MQ=255)                                                aGCGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGcc                           >  1:578095/1‑81 (MQ=255)                                                  cGTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGt                         <  1:578043/81‑1 (MQ=255)                                                   gTCATCAACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTg                        <  1:578044/81‑1 (MQ=255)                                                      atcaACAATGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTggg                      <  1:578045/80‑1 (MQ=255)                                                         aacaaTGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAAt                   >  1:578085/1‑80 (MQ=255)                                                           caaTGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGa                 >  2:578098/1‑80 (MQ=255)                                                            aaTGCCAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGac                >  2:578089/1‑80 (MQ=255)                                                                ccAGCGCCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGacaaac            <  2:578046/80‑1 (MQ=255)                                                                   gcgcCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGc        <  2:578047/81‑1 (MQ=255)                                                                    cgcCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCa       >  1:578090/1‑81 (MQ=255)                                                                     gcCGGAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCAt      <  2:578048/81‑1 (MQ=255)                                                                         gAGTGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTg  <  2:578049/81‑1 (MQ=255)                                                                            |                                                                              GGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATCGGAATTTCTGCTGCCGTGGGAATGACAAACAAGCATCCTG  >  NC_000913/3297178‑3297330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑