Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,307,326	C→T	E179K (GAA→AAA)	deaD ←	ATP‑dependent RNA helicase DeaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,307,326	0	C	T	100.0%	115.7 / NA	34	E179K (GAA→AAA)	deaD	ATP‑dependent RNA helicase DeaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/15);  total (19/15)

GCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATCCAGAACCA  >  NC_000913/3307248‑3307403                                                                               |                                                                              gCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCg                                                                             >  2:580333/1‑81 (MQ=255)     tttCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCggg                                                                           <  2:580288/79‑1 (MQ=255)         aTAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTgc                                                                     <  1:580289/81‑1 (MQ=255)         aTAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTgc                                                                     >  2:580366/1‑81 (MQ=255)           aaaGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTgcgc                                                                   >  2:580337/1‑81 (MQ=255)             aGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCa                                                                 >  1:580331/1‑81 (MQ=255)                           cgacgaATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACg                                                   >  2:580336/1‑81 (MQ=255)                            gacgaATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGt                                                  >  2:580335/1‑81 (MQ=255)                            gacgaATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGt                                                  >  2:580329/1‑81 (MQ=255)                               gaATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCtt                                               >  2:580341/1‑81 (MQ=255)                                aaTCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTc                                              <  2:580290/81‑1 (MQ=255)                                     cTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGa                                         <  1:580292/81‑1 (MQ=255)                                     cTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGa                                         <  2:580291/81‑1 (MQ=255)                                      ttCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATg                                          >  2:580328/1‑79 (MQ=255)                                        ccGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAg                                       <  1:580293/80‑1 (MQ=255)                                        ccGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGc                                      >  2:580326/1‑81 (MQ=255)                                          ggCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGcc                                     >  1:580340/1‑80 (MQ=255)                                            cATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCAt                                  >  2:580330/1‑81 (MQ=255)                                             aTGGTTGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATg                                 >  1:580346/1‑81 (MQ=255)                                                 ttGCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATgcg                               >  1:580356/1‑79 (MQ=255)                                                   gCAGAGAACAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCgcag                            <  2:580294/80‑1 (MQ=255)                                                          aCAGAGCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGt                    <  1:580295/81‑1 (MQ=255)                                                               gCGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCt               <  1:580296/81‑1 (MQ=255)                                                                cGGTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCtt              <  1:580297/81‑1 (MQ=255)                                                                 ggTCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCtt              >  1:580332/1‑80 (MQ=255)                                                                   tCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCAt           <  2:580298/81‑1 (MQ=255)                                                                   tCTGATGACCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCAt           <  1:580299/81‑1 (MQ=255)                                                                      gatgaCCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATc          >  1:580350/1‑79 (MQ=255)                                                                       atgaCCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATccg        >  2:580364/1‑79 (MQ=255)                                                                       atgaCCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATcc         <  2:580300/79‑1 (MQ=255)                                                                       atgaCCTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATcc         <  1:580301/79‑1 (MQ=255)                                                                           ccTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATCCAGAAc    >  2:580302/1‑80 (MQ=255)                                                                            cTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATCCAGAAc    >  2:580360/1‑79 (MQ=255)                                                                            cTTTCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATCCAGAACCa  <  2:580303/81‑1 (MQ=255)                                                                               |                                                                              GCTCTTTCATAAAGCGGCGGGTAATGCGACGAATCGCTTCCGGCATGGTTGCAGAGAACAGAGCGGTCTGATGACCTTCCGGGATCTGCGCCATAATGGTTTCAACGTCTTCGATGAAGCCCATGCGCAGCATTTCGTCAGCTTCATCCAGAACCA  >  NC_000913/3307248‑3307403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑