Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,332,210	C→A	V176L (GTG→TTG)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,210	0	C	A	100.0%	88.4 / NA	26	V176L (GTG→TTG)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/13);  total (13/13)

CGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATA  >  NC_000913/3332133‑3332287                                                                              |                                                                              cgcAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcc                                                                            <  2:585509/81‑1 (MQ=255)   cAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGcgccaagcca                                                                           >  2:585538/1‑80 (MQ=255)          aCATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCAc                                                                   >  1:585537/1‑81 (MQ=255)             tAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCa                                                                <  2:585510/81‑1 (MQ=255)                aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAc                                                             <  1:585512/81‑1 (MQ=255)                aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAAc                                                             <  1:585511/81‑1 (MQ=255)                  tgtACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCg                                                           <  2:585513/81‑1 (MQ=255)                    tACAGTAAAAACAGGCTCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTc                                                         <  2:585514/81‑1 (MQ=255)                     aCAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTcg                                                        >  2:585542/1‑81 (MQ=255)                      cAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTcg                                                        <  1:585515/80‑1 (MQ=255)                            aaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCAcc                                                 <  2:585516/81‑1 (MQ=255)                                aGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAcc                                             <  2:585517/81‑1 (MQ=255)                                  gATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAcccc                                           <  1:585518/81‑1 (MQ=255)                                   aTCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGAcccc                                           >  2:585545/1‑80 (MQ=255)                                        cGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGa                                      <  1:585519/80‑1 (MQ=255)                                         ggTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGAt                                     >  1:585543/1‑80 (MQ=255)                                             aGGCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACt                                 <  2:585520/80‑1 (MQ=255)                                              ggCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTcc                               >  2:585547/1‑81 (MQ=255)                                               gCCAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTccc                              >  2:585554/1‑81 (MQ=255)                                                 cAGCCGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTccc                              >  1:585553/1‑79 (MQ=255)                                                    ccGACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgt                         >  2:585551/1‑81 (MQ=255)                                                      gACGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGgtg                        >  1:585550/1‑80 (MQ=255)                                                        cGCAATAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTaa                     >  2:585560/1‑81 (MQ=255)                                                           aaTAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCg                  <  2:585521/81‑1 (MQ=255)                                                            aTAAAACCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCgg                 <  1:585522/81‑1 (MQ=255)                                                               aaaCCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGt                >  1:585559/1‑79 (MQ=255)                                                                aaCCTTTTGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACAGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAg             >  1:585556/1‑81 (MQ=255)                                                                      ttGCGCCAAGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTg        >  2:585546/1‑80 (MQ=255)                                                                           ccaagccaTAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAata  >  2:585555/1‑81 (MQ=255)                                                                              |                                                                              CGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATA  >  NC_000913/3332133‑3332287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑