Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,351,386	T→C	K547R (AAA→AGA)	arcB ←	sensory histidine kinase ArcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,351,386	0	T	C	100.0%	93.4 / NA	27	K547R (AAA→AGA)	arcB	sensory histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

ACCAGGTCGTATTCGCCCGGTTTAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAG  >  NC_000913/3351308‑3351461                                                                               |                                                                            acCAGGTCGTATTCGCCCGGTTTAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAAtctt                                                                           >  2:589638/1‑81 (MQ=255)    aGGTCGTATTCGCCCGGTTTAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTc                                                                         >  2:589629/1‑80 (MQ=255)            ttCGCCCGGTTTAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAg                                                                 >  2:589634/1‑80 (MQ=255)            ttCGCCCGGTTTAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAg                                                                 >  1:589644/1‑80 (MQ=255)                 ccGGTTTAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAAcgcg                                                           >  2:589622/1‑81 (MQ=255)                     tttAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGcaac                                                       <  1:589585/81‑1 (MQ=255)                       tAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGcaaca                                                      <  2:589586/80‑1 (MQ=255)                        aaaCATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAAt                                                    >  2:589637/1‑81 (MQ=255)                         aaCATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAAt                                                    >  1:589623/1‑80 (MQ=255)                          aCATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCa                                                  >  1:589652/1‑81 (MQ=255)                                 cAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACgttc                                             <  2:589587/79‑1 (MQ=255)                                 cAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACgttc                                             <  2:589588/79‑1 (MQ=255)                                  aGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACgttcagt                                          >  2:589646/1‑81 (MQ=255)                                    cgccgcCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACgttcagtt                                         >  2:589636/1‑80 (MQ=255)                                      ccgccTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCaa                                      <  1:589589/81‑1 (MQ=255)                                      ccgccTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCaa                                      >  1:589643/1‑81 (MQ=255)                                        gccTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATg                                    <  2:589590/81‑1 (MQ=255)                                        gccTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATg                                    >  1:589649/1‑81 (MQ=255)                                        gccTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATg                                    >  2:589627/1‑81 (MQ=255)                                           ttGCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCt                                 >  1:589650/1‑81 (MQ=255)                                             gCCGGTCATGGCGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTc                               >  2:589635/1‑81 (MQ=255)                                                        cGACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAgcagca                    <  1:589591/81‑1 (MQ=255)                                                         gACATCAACGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGcac                   <  2:589592/81‑1 (MQ=255)                                                               aaCGCTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAg             <  2:589593/81‑1 (MQ=255)                                                                   cTGTTACCTAATCTTTCCAGCATAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGcc         >  2:589655/1‑81 (MQ=255)                                                                   cTGTTACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGcc         <  1:589594/81‑1 (MQ=255)                                                                      ttACCTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGt      <  2:589595/81‑1 (MQ=255)                                                                          cTAATCTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAg  <  2:589596/81‑1 (MQ=255)                                                                               |                                                                            ACCAGGTCGTATTCGCCCGGTTTAAACATCTCCAGCGCCGCCTTGCCGGTCATGGCGACATCAACGCTGTTACCTAATTTTTCCAGCACAGAACGCGCAACAATCACGTTCAGTTCAATGTCTTCCACCAGCAGCACATTCAGCGCCGGTAAAG  >  NC_000913/3351308‑3351461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑