Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,775	C→T	intergenic (‑286/‑94)	nanR ← / → dcuD	DNA‑binding transcriptional dual regulator NanR/putative transporter DcuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,775	0	C	T	100.0%	48.8 / NA	15	intergenic (‑286/‑94)	nanR/dcuD	DNA‑binding transcriptional dual regulator NanR/putative transporter DcuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/3);  total (12/3)

ATCATAAGCGCATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTC  >  NC_000913/3374715‑3374848                                                             |                                                                          atCATAAGCGCATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCa                                                       <  1:594349/81‑1 (MQ=255)   cATAAGCGCATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCaa                                                      >  2:594363/1‑80 (MQ=255)      aaGCGCATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCaaaaaa                                                  >  1:594350/1‑81 (MQ=255)                         aaaaaTCACCTCACCTACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTc                                >  1:594371/1‑80 (MQ=255)                           aaaTCACCTCACCTACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAAt                             >  1:594367/1‑81 (MQ=255)                              tcacctcaccTACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTg                          >  1:594374/1‑81 (MQ=255)                                acctcacctACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGcc                        >  1:594373/1‑81 (MQ=255)                                     acctACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTa                   >  2:594369/1‑81 (MQ=255)                                      cctACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTAt                  <  1:594351/81‑1 (MQ=255)                                       ctACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATg                 >  1:594370/1‑81 (MQ=255)                                       ctACAATTAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATg                 >  2:594368/1‑81 (MQ=255)                                              tAAAAACACGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGa          >  2:594365/1‑81 (MQ=255)                                                     acGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGtttt   >  2:594377/1‑81 (MQ=255)                                                     acGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGtttt   >  2:594378/1‑81 (MQ=255)                                                      cGACATCTGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTc  <  1:594352/81‑1 (MQ=255)                                                             |                                                                          ATCATAAGCGCATATTTTTTAATGAAAAATCACCTCACCTACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTC  >  NC_000913/3374715‑3374848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑