Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,397,596	A→T	F67Y (TTT→TAT)	rng ←	RNase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,397,596	0	A	T	100.0%	67.6 / NA	20	F67Y (TTT→TAT)	rng	RNase G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/7);  total (13/7)

GTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTG  >  NC_000913/3397530‑3397670                                                                   |                                                                           gTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATcc                                                              <  1:598977/81‑1 (MQ=255) gTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATcc                                                              <  1:598976/81‑1 (MQ=255)     aTTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGcc                                                          >  2:599005/1‑81 (MQ=255)          ttttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAata                                                     >  1:598996/1‑81 (MQ=255)          ttttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAata                                                     >  2:598993/1‑81 (MQ=255)             tGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATc                                                   >  1:598994/1‑80 (MQ=255)                    cACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAg                                           >  2:598998/1‑81 (MQ=255)                       ccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCAGCTTTATCCAGCCCAATATCTACAAAAgccg                                        <  2:598978/81‑1 (MQ=255)                            acacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGc                                   <  1:598979/81‑1 (MQ=255)                              acaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCAt                                 <  1:598980/81‑1 (MQ=255)                                aTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAc                               >  1:599008/1‑81 (MQ=255)                                aTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAc                               >  1:598997/1‑81 (MQ=255)                                 ttCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAcc                              >  1:599000/1‑81 (MQ=255)                                   cGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCg                            >  2:599011/1‑81 (MQ=255)                                        tgCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGt                       <  1:598981/81‑1 (MQ=255)                                           ggCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTaca                    >  1:599016/1‑81 (MQ=255)                                            gCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTacac                   >  2:599009/1‑81 (MQ=255)                                               tgatgTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGAc                <  2:598982/81‑1 (MQ=255)                                                   gTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTa             >  2:599014/1‑80 (MQ=255)                                                           cATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCt    >  2:599006/1‑81 (MQ=255)                                                              gaagatACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTg  >  1:599010/1‑80 (MQ=255)                                                                   |                                                                           GTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTG  >  NC_000913/3397530‑3397670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑