Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,418,852	G→A	intergenic (+241/‑190)	yhdV → / → yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,418,852	0	G	A	100.0%	69.3 / NA	21	intergenic (+241/‑190)	yhdV/yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/11);  total (10/11)

AGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGC  >  NC_000913/3418780‑3418927                                                                         |                                                                            aGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAATTGTGACGAATGtt                                                                     <  2:603177/81‑1 (MQ=255)              cGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCAcc                                                        <  2:603178/81‑1 (MQ=255)                   aCAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTg                                                    <  1:603179/80‑1 (MQ=255)                      aTCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCggg                                                <  1:603180/81‑1 (MQ=255)                            attCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCt                                           <  1:603181/80‑1 (MQ=255)                            attCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTg                                          >  1:603219/1‑81 (MQ=255)                              tCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGtt                                        >  1:603204/1‑81 (MQ=255)                               cTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGttt                                       >  2:603203/1‑81 (MQ=255)                                 gCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGtttt                                      >  1:603211/1‑80 (MQ=255)                                   ttAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTttat                                   >  1:603220/1‑81 (MQ=255)                                      gTAAAACACGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTttatta                                 <  1:603182/80‑1 (MQ=255)                                           acacGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCt                           <  1:603183/81‑1 (MQ=255)                                             acGCGTAAATATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGt                         >  1:603218/1‑81 (MQ=255)                                                   aaaTATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTacac                   >  1:603205/1‑81 (MQ=255)                                                    aaTATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACt                  <  1:603184/81‑1 (MQ=255)                                                    aaTATTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACt                  >  2:603206/1‑81 (MQ=255)                                                     atatTTTGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACt                  >  2:603208/1‑80 (MQ=255)                                                           tGCGCCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCtt            <  1:603185/80‑1 (MQ=255)                                                            gcgcCAAAATGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTAt          >  2:603213/1‑81 (MQ=255)                                                                   aaTGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGAcg   <  1:603186/81‑1 (MQ=255)                                                                    aTGTGACGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGAcgc  <  1:603187/81‑1 (MQ=255)                                                                         |                                                                            AGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGC  >  NC_000913/3418780‑3418927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑