| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,454,025 | T→A | S458S (TCA→TCT) | gspA ← | Type II secretion system protein GspA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,454,025 | 0 | T | A | 100.0% | 56.9 / NA | 17 | S458S (TCA→TCT) | gspA | Type II secretion system protein GspA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/7); total (10/7) | |||||||||||
CCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTATTTTTTCTACCAACAAGGGACG > NC_000913/3453952‑3454102 | ccGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGc > 1:610137/1‑81 (MQ=255)ccGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGc > 2:610130/1‑81 (MQ=255) gAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGCcaac > 1:610126/1‑80 (MQ=255) tttGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGCCAACAACAcc < 1:610112/80‑1 (MQ=255) tataAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAgtgg > 1:610143/1‑81 (MQ=255) aGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGc > 2:610136/1‑81 (MQ=255) aGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGc < 2:610113/81‑1 (MQ=255) cACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCtttttt > 2:610135/1‑81 (MQ=255) ctcCCCTGCCACCTGCCAGAGATGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGaaa > 2:610139/1‑81 (MQ=255) ccacctgccaGAGATGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCtt < 2:610114/79‑1 (MQ=255) ccacctgccaGAGATGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTa < 1:610115/81‑1 (MQ=255) ctgccaGAGATGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTAtttt < 2:610116/81‑1 (MQ=255) agaTGTACTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTATTTTTTCTAc > 2:610142/1‑80 (MQ=255) cTAACGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTATTTTTTCTACCAACAAgg > 1:610147/1‑81 (MQ=255) aCGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTATTTTTTCTACCAACAAGGGAc < 1:610118/81‑1 (MQ=255) aCGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTATTTTTTCTACCAACAAGGGAc < 1:610117/81‑1 (MQ=255) cGTAGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTATTTTTTCTACCAACAAGGGACg > 2:610154/1‑81 (MQ=255) | CCGGGGAAATATTTGCTTCATCCCGATATAAGTAGGCACTCTCCCCTGCCACCTGCCAGAGATGTACTAACGTTGAAAAGCCAACAACACCGTCAGTTTTCAAGTGGTGGCTTTTTTGAAATTGCTTTATTTTTTCTACCAACAAGGGACG > NC_000913/3453952‑3454102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |