Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,692	G→T	A608E (GCG→GAG)	fusA ←	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,692	0	G	T	100.0%	94.8 / NA	28	A608E (GCG→GAG)	fusA	elongation factor G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/11);  total (17/11)

ACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCA  >  NC_000913/3471616‑3471763                                                                             |                                                                        aCCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCttt                                                                     >  2:613510/1‑81 (MQ=255)   cGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCt                                                                   >  2:613512/1‑81 (MQ=255)      tAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTaa                                                                <  1:613471/81‑1 (MQ=255)        aCGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAg                                                              >  1:613508/1‑81 (MQ=255)         cGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGc                                                             <  2:613472/81‑1 (MQ=255)         cGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGc                                                             <  2:613473/81‑1 (MQ=255)          gTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCCCAAGCAGAACTGGTTTCTCTTTCTTAAAGcc                                                            >  2:613499/1‑81 (MQ=255)            cACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCctt                                                          >  2:613503/1‑81 (MQ=255)             aCCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCcttc                                                         >  1:613509/1‑81 (MQ=255)                ggTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCcttctt                                                       >  2:613511/1‑80 (MQ=255)                    ttCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAg                                                  >  1:613507/1‑81 (MQ=255)                          tCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGa                                              >  2:613524/1‑79 (MQ=255)                           ccGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGAt                                             <  2:613474/79‑1 (MQ=255)                               aGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAagc                                       <  2:613475/81‑1 (MQ=255)                                       cTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGt                                <  1:613476/80‑1 (MQ=255)                                          cAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTa                             <  2:613477/80‑1 (MQ=255)                                           aaCCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTaaa                           >  2:613521/1‑81 (MQ=255)                                            aCCTTCATGATCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAAc                          >  2:613495/1‑81 (MQ=255)                                                     aTCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGtt                   >  2:613520/1‑79 (MQ=255)                                                     aTCGGCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTc                  <  2:613478/80‑1 (MQ=255)                                                        ggCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag              >  1:613528/1‑81 (MQ=255)                                                        ggCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCagag              >  1:613526/1‑81 (MQ=255)                                                         gCTCAAGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAgagg             <  2:613479/81‑1 (MQ=255)                                                             aaGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGt          <  1:613480/80‑1 (MQ=255)                                                             aaGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTc         <  2:613481/81‑1 (MQ=255)                                                              aGCAGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTc         >  1:613514/1‑80 (MQ=255)                                                                 aGAACTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACg     >  2:613519/1‑81 (MQ=255)                                                                    aCTGGTTTCTCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCa  >  1:613517/1‑81 (MQ=255)                                                                             |                                                                        ACCGATAACGTCACCGGTGTTCTCTTCCGGAGTTTCTACTTCAACCTTCATGATCGGCTCAAGCAGAACTGGTTTCGCTTTCTTAAAGCCTTCTTTAAAGGCGATAGAAGCAGCCAGTTTAAACGCCAGTTCAGAGGAGTCAACGTCA  >  NC_000913/3471616‑3471763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑