Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,497,005	G→A	M1M (ATG→ATA) †	nirC →	nitrite transporter NirC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,497,005	0	G	A	100.0%	49.4 / NA	15	M1M (ATG→ATA) †	nirC	nitrite transporter NirC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/3);  total (12/3)

AATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTT  >  NC_000913/3496927‑3497082                                                                               |                                                                              aatCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATAtt                                                                             >  1:618998/1‑81 (MQ=255)                 cAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATtaa                                                              >  2:618992/1‑80 (MQ=255)                                      tGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTgc                                         <  1:618983/80‑1 (MQ=255)                                         tttATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTgcgcg                                      >  1:618997/1‑80 (MQ=255)                                               ttttGTTTTTATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGc                                <  2:618984/80‑1 (MQ=255)                                                       ttATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTc                       >  1:619006/1‑81 (MQ=255)                                                       ttATTTTTTAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTc                       >  2:618996/1‑81 (MQ=255)                                                              ttAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCaaa                 >  1:618995/1‑80 (MQ=255)                                                              ttAAAGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCaaa                 >  1:619003/1‑80 (MQ=255)                                                                aaaGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATaa              >  2:619007/1‑81 (MQ=255)                                                                  aGGATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAAcc            >  2:618994/1‑81 (MQ=255)                                                                    gATAATCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCg          >  2:619000/1‑81 (MQ=255)                                                                        aTCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCg      <  1:618985/81‑1 (MQ=255)                                                                         tCAAATATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCgg     >  1:618999/1‑81 (MQ=255)                                                                            aaTATTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCtt  >  1:618993/1‑81 (MQ=255)                                                                               |                                                                              AATCTTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTTATTTTTTAAAGGATAATCAAATGTTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTT  >  NC_000913/3496927‑3497082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑