Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,512,195	C→T	L50L (TTG→TTA)	yhfZ ←	putative DNA‑binding transcriptional regulator YhfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,512,195	0	C	T	100.0%	75.1 / NA	22	L50L (TTG→TTA)	yhfZ	putative DNA‑binding transcriptional regulator YhfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/15);  total (7/15)

TCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACC  >  NC_000913/3512121‑3512269                                                                           |                                                                           tCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAAcgc                                                                       <  1:622178/80‑1 (MQ=255)         gACCAGATAACTGCCATTGCGCCCACGGCGATCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCg                                                               <  2:622179/80‑1 (MQ=255)          aCCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGt                                                              >  2:622217/1‑80 (MQ=255)          aCCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGt                                                              >  1:622214/1‑80 (MQ=255)              gATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGc                                                         <  1:622180/81‑1 (MQ=255)               aTAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGc                                                         >  1:622195/1‑80 (MQ=255)                       cATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATg                                                <  2:622181/81‑1 (MQ=255)                         ttGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGaa                                              >  2:622215/1‑81 (MQ=255)                           gcgcCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAAc                                             <  2:622182/80‑1 (MQ=255)                           gcgcCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAAc                                             <  1:622183/80‑1 (MQ=255)                             gcCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGc                                          <  1:622184/81‑1 (MQ=255)                                          aaTCCGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAg                             >  1:622216/1‑81 (MQ=255)                                              cGTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTc                          <  1:622185/80‑1 (MQ=255)                                               gTATCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCAt                        <  2:622186/81‑1 (MQ=255)                                                  tCGCTCCGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCt                      <  2:622187/80‑1 (MQ=255)                                                       ccGCTTGATTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGtt                >  2:622220/1‑81 (MQ=255)                                                              aTTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAAt          <  1:622189/80‑1 (MQ=255)                                                              aTTCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAAt          <  2:622188/80‑1 (MQ=255)                                                               ttCCAGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTCTCAATCg        <  2:622190/81‑1 (MQ=255)                                                                   aGCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTa    >  2:622222/1‑81 (MQ=255)                                                                    gCGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTAc   <  1:622191/81‑1 (MQ=255)                                                                     cGTTTTTAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTAcc  <  1:622192/81‑1 (MQ=255)                                                                           |                                                                           TCCATCTCGACCAGATAACTGCCATTGCGCCCACGGCGTTCAATCCGTATCGCTCCGCTTGATTCCAGCGTTTTCAACGCGGCCTGCGTCAGGCCAACGGATGAACGGCATTCATTTGCCAGCTCATCTATGGTTTTCAATCGATTACC  >  NC_000913/3512121‑3512269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑