Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,601,119	C→T	C323Y (TGC→TAC)	ftsX ←	cell division protein FtsX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,601,119	0	C	T	100.0%	75.3 / NA	23	C323Y (TGC→TAC)	ftsX	cell division protein FtsX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/13);  total (10/13)

GTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGC  >  NC_000913/3601048‑3601186                                                                        |                                                                    gTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGa                                                            <  2:641462/81‑1 (MQ=255)              cGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCAtt                                               <  1:641463/81‑1 (MQ=255)               gTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTg                                              <  2:641464/81‑1 (MQ=255)                   cAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGatat                                          >  2:641484/1‑81 (MQ=255)                         aCGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACt                                    >  2:641504/1‑81 (MQ=255)                         aCGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACt                                    <  2:641465/81‑1 (MQ=255)                           gCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTc                                  >  2:641502/1‑81 (MQ=255)                            cTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCg                                 <  1:641466/81‑1 (MQ=255)                             tGCCACCCAGCCAATCATCGAGCGTACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGt                                >  1:641494/1‑81 (MQ=255)                             tGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGt                                >  2:641516/1‑81 (MQ=255)                               caaCCCAGCCAATCATCGAGCATACCAGCAGCAATAGGAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGtt                               <  1:641467/78‑1 (MQ=255)                                   ccAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGaa                          <  2:641468/81‑1 (MQ=255)                                       ccAATCATCGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAAcc                      <  1:641469/81‑1 (MQ=255)                                               cGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCAc              >  1:641499/1‑81 (MQ=255)                                               cGAGCATACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCAc              >  1:641500/1‑81 (MQ=255)                                                    aTACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCg         >  2:641508/1‑81 (MQ=255)                                                     tACCAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCg         >  2:641491/1‑80 (MQ=255)                                                       ccAGCAGCAATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCaa      <  2:641470/81‑1 (MQ=255)                                                           cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc  >  1:641498/1‑81 (MQ=255)                                                           cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc  <  1:641474/81‑1 (MQ=255)                                                           cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc  <  1:641473/81‑1 (MQ=255)                                                           cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc  <  2:641472/81‑1 (MQ=255)                                                           cagcaATAGCAGGTATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAAccgc  <  1:641471/81‑1 (MQ=255)                                                                        |                                                                    GTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAACCTGTGCCACTTCCGCAACCGC  >  NC_000913/3601048‑3601186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑