Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,602,830	C→T	G472S (GGT→AGT)	ftsY ←	signal recognition particle receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,602,830	0	C	T	100.0%	108.2 / NA	31	G472S (GGT→AGT)	ftsY	signal recognition particle receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

ATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTT  >  NC_000913/3602753‑3602905                                                                              |                                                                            aTCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAAt                                                                          <  1:641872/81‑1 (MQ=255)   ccTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATg                                                                         >  2:641926/1‑80 (MQ=255)    ctctCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTa                                                                       <  1:641873/81‑1 (MQ=255)    ctctCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTa                                                                       <  2:641874/81‑1 (MQ=255)      ctcGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGc                                                                     >  2:641928/1‑81 (MQ=255)            cAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAg                                                               <  2:641875/81‑1 (MQ=255)             aaaaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAgg                                                              <  2:641876/81‑1 (MQ=255)              aaaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAgg                                                              >  1:641914/1‑80 (MQ=255)               aaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGa                                                            <  1:641877/81‑1 (MQ=255)               aaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGa                                                            <  1:641878/81‑1 (MQ=255)                  gTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATAc                                                         >  2:641930/1‑81 (MQ=255)                              aGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAg                                             >  2:641921/1‑81 (MQ=255)                                 cgtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCa                                          >  2:641946/1‑81 (MQ=255)                                  gtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCAc                                         >  2:641945/1‑81 (MQ=255)                                  gtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCAc                                         <  2:641879/81‑1 (MQ=255)                                       ccTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCgaga                                    <  2:641880/81‑1 (MQ=255)                                       ccTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCgag                                     >  1:641947/1‑80 (MQ=255)                                           aaaCGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAAt                                >  2:641931/1‑81 (MQ=255)                                           aaaCGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAAt                                >  2:641934/1‑81 (MQ=255)                                           aaaCGGACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAAt                                >  2:641924/1‑81 (MQ=255)                                                gACGCAAATCCTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTAccc                           <  2:641881/81‑1 (MQ=255)                                                         ccTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCg                  >  2:641951/1‑81 (MQ=255)                                                          cTCAATACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCgc                 >  2:641935/1‑81 (MQ=255)                                                             aaTACGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCgcc                <  2:641882/79‑1 (MQ=255)                                                                aCGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCgccgtgcc           >  1:641932/1‑81 (MQ=255)                                                                 cGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCgccgtgccg          >  2:641929/1‑81 (MQ=255)                                                                 cGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCgccgtgccg          <  2:641883/81‑1 (MQ=255)                                                                 cGTTCGCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCgccgtgccg          >  2:641933/1‑81 (MQ=255)                                                                      gCCGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAg     <  2:641884/81‑1 (MQ=255)                                                                        cGACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGtt   >  1:641918/1‑81 (MQ=255)                                                                         gACACTAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGttt  <  1:641885/81‑1 (MQ=255)                                                                              |                                                                            ATCCTCTCGGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTT  >  NC_000913/3602753‑3602905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑