Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,608,490	C→T	A680A (GCC→GCT)	zntA →	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,608,490	0	C	T	100.0%	97.8 / NA	29	A680A (GCC→GCT)	zntA	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/10);  total (19/10)

GCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGG  >  NC_000913/3608416‑3608567                                                                           |                                                                              gccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATc                                                                         >  1:643202/1‑81 (MQ=255) gccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATc                                                                         <  1:643163/81‑1 (MQ=255)    gACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGc                                                                      >  2:643210/1‑81 (MQ=255)     aCGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGc                                                                      >  2:643199/1‑80 (MQ=255)           caTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACa                                                               >  1:643201/1‑81 (MQ=255)                aCCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACt                                                          <  1:643164/81‑1 (MQ=255)                 cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTa                                                         >  2:643205/1‑81 (MQ=255)                 cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTa                                                         >  1:643208/1‑81 (MQ=255)                      aaCCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTgcg                                                    >  2:643200/1‑81 (MQ=255)                        ccaccTGCGCGGCCTGGTGCAAATGATTGAGCTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCt                                                  <  1:643165/81‑1 (MQ=255)                         caccTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTg                                                 >  1:643193/1‑81 (MQ=255)                          accTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTgg                                                >  1:643203/1‑81 (MQ=255)                                  ggCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa                                          >  1:643213/1‑79 (MQ=255)                                  ggCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa                                          >  2:643206/1‑79 (MQ=255)                                       ggTGCAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGAt                                   >  1:643240/1‑81 (MQ=255)                                           cAAATGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTc                               <  2:643166/81‑1 (MQ=255)                                              aTGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTc                            <  1:643167/81‑1 (MQ=255)                                               tGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCg                           >  1:643216/1‑81 (MQ=255)                                               tGATTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCg                           <  1:643168/81‑1 (MQ=255)                                                 aTTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGt                          >  1:643212/1‑80 (MQ=255)                                                 aTTGAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTc                         <  1:643169/81‑1 (MQ=255)                                                    gAACTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcac                       >  2:643224/1‑80 (MQ=255)                                                      aCTGGCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcaccac                    <  1:643170/81‑1 (MQ=255)                                                          gCACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTg                >  1:643214/1‑81 (MQ=255)                                                           cACGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGt               >  1:643215/1‑81 (MQ=255)                                                            aCGCGCCACTCACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGtt              <  2:643171/81‑1 (MQ=255)                                                                      cACGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGAcc    >  2:643217/1‑81 (MQ=255)                                                                       aCGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGAcc    <  1:643172/80‑1 (MQ=255)                                                                        cGCTAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCgg  >  1:643218/1‑81 (MQ=255)                                                                           |                                                                              GCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGG  >  NC_000913/3608416‑3608567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑