Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,674,509	A→T	intergenic (+134/‑277)	yhjD → / → yhjE	putative transporter YhjD/putative transporter YhjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,674,509	0	A	T	100.0%	23.2 / NA	8	intergenic (+134/‑277)	yhjD/yhjE	putative transporter YhjD/putative transporter YhjE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (3/5);  total (3/5)

CCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGT  >  NC_000913/3674434‑3674581                                                                            |                                                                         ccccGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCTGAGcc                                                                     >  1:655863/1‑81 (MQ=255)      tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCTGAGCCTCTTc                                                                <  1:655843/81‑1 (MQ=255)                                gcatcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCTGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAAtt                                      <  2:655844/81‑1 (MQ=255)                                     cGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCTGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCAtt                                 >  2:655861/1‑81 (MQ=255)                                           taATGCGCACATAACCTCTTGAAACTCGTCCCCTGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACt                           <  1:655845/81‑1 (MQ=255)                                              tGCGCACATAACCTCTTGAAACTCGTCCCCTGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCAt                        <  1:655846/81‑1 (MQ=255)                                                               gAAACTCGTCCCCTGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCAtata          <  2:655847/78‑1 (MQ=255)                                                                    tCGTCCCCTGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGt  >  1:655862/1‑81 (MQ=255)                                                                            |                                                                         CCCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGT  >  NC_000913/3674434‑3674581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑