Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,699,322	T→A	V370E (GTG→GAG)	bcsG →	cellulose phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,699,322	0	T	A	100.0%	83.4 / NA	25	V370E (GTG→GAG)	bcsG	cellulose phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/9);  total (16/9)

GAATTGATGGATCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGTGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTCTACAACACGCTTCCACT  >  NC_000913/3699246‑3699397                                                                             |                                                                            gAATTGATGGATCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCt                                                                          >  1:661479/1‑80 (MQ=255)      gATGGATCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAAcc                                                                     <  1:661427/80‑1 (MQ=255)         ggATCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCgctg                                                                 >  2:661458/1‑81 (MQ=255)          gATCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCgctgg                                                                >  2:661465/1‑81 (MQ=255)          gATCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCgctg                                                                 <  1:661428/80‑1 (MQ=255)           aTCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCgctggc                                                               >  1:661457/1‑81 (MQ=255)            tCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCgctggct                                                              >  1:661451/1‑81 (MQ=255)                acaaaTCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGAc                                                          >  1:661452/1‑81 (MQ=255)                   aaTCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGtt                                                       <  2:661429/81‑1 (MQ=255)                       tGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTAcc                                                    >  1:661475/1‑80 (MQ=255)                               aTTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGa                                            >  2:661476/1‑80 (MQ=255)                                ttttGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGAt                                           >  2:661450/1‑80 (MQ=255)                                       gggCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAg                                   >  1:661459/1‑81 (MQ=255)                                        ggCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGc                                  <  1:661430/81‑1 (MQ=255)                                             tGATGGTTCGCCGGTTTATGACGATACCGCTGAGCTAAACCGCTGGCTAGACGTTACCGAAAAAGGTAAAAACAGCCGTa                              <  2:661431/80‑1 (MQ=255)                                                 ggTTCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGc                          >  1:661460/1‑80 (MQ=255)                                                   ttCGCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCAc                       <  2:661432/81‑1 (MQ=255)                                                      gCCGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGtt                    >  2:661467/1‑81 (MQ=255)                                                       ccGGTTTATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTc                   >  1:661477/1‑81 (MQ=255)                                                            ttATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTCTacaa              >  1:661469/1‑81 (MQ=255)                                                            ttATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTCTacaa              >  2:661468/1‑81 (MQ=255)                                                             tATGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTCTacaa              >  2:661455/1‑80 (MQ=255)                                                              aTGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTCTacaac             <  2:661433/80‑1 (MQ=255)                                                               tGACGATACCGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTCTACAacac           <  1:661434/81‑1 (MQ=255)                                                                        cGCTGAGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTCTACAACACGCTTCCACt  <  2:661435/81‑1 (MQ=255)                                                                             |                                                                            GAATTGATGGATCAAACAAATCTGCCGGTTATTTTGCTGGGCTTTGATGGTTCGCCGGTTTATGACGATACCGCTGTGCTTAACCGCTGGCTGGACGTTACCGAAAAAGATAAAAACAGCCGTAGTGCCACGTTCTACAACACGCTTCCACT  >  NC_000913/3699246‑3699397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑