Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,740,658	C→A	I318I (ATC→ATA)	avtA →	valine‑‑pyruvate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,740,658	0	C	A	100.0%	96.9 / NA	28	I318I (ATC→ATA)	avtA	valine‑‑pyruvate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/17);  total (11/17)

TTAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAT  >  NC_000913/3740582‑3740734                                                                             |                                                                             ttAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCca                                                                          >  2:669578/1‑81 (MQ=255)    aGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCcatc                                                                        <  2:669579/80‑1 (MQ=255)     gCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCcatcat                                                                      <  1:669580/81‑1 (MQ=255)      cGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCAtt                                                                     >  2:669616/1‑81 (MQ=255)       gTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTc                                                                    >  1:669627/1‑81 (MQ=255)       gTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTc                                                                    <  2:669581/81‑1 (MQ=255)        tAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcg                                                                   <  2:669582/81‑1 (MQ=255)         aaCGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgc                                                                  >  1:669618/1‑81 (MQ=255)          aCGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgcc                                                                 >  1:669610/1‑81 (MQ=255)                  ctgcGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTAttt                                                          <  2:669583/80‑1 (MQ=255)                                aaCAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTg                                           <  2:669584/81‑1 (MQ=255)                                 aCAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGc                                          >  1:669624/1‑81 (MQ=255)                                    gTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTg                                       <  2:669585/81‑1 (MQ=255)                                       atcaAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGAt                                     <  1:669586/80‑1 (MQ=255)                                             ccGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATaaa                              <  2:669587/81‑1 (MQ=255)                                                  ttACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCgg                          <  2:669588/80‑1 (MQ=255)                                                     ctacCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAgg                      <  2:669589/81‑1 (MQ=255)                                                             gtgtTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCAttt              <  1:669591/81‑1 (MQ=255)                                                             gtgtTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCAttt              <  1:669590/81‑1 (MQ=255)                                                             gtgtTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCAttt              >  2:669630/1‑81 (MQ=255)                                                                ttCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTAATTCATAAACCGGAAGGAGCCATTTTcc           <  2:669592/81‑1 (MQ=255)                                                                     gAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTgg      >  1:669635/1‑81 (MQ=255)                                                                     gAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTgg      >  1:669621/1‑81 (MQ=255)                                                                     gAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTgg      <  2:669593/81‑1 (MQ=255)                                                                        aCTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTa   >  1:669626/1‑81 (MQ=255)                                                                        aCTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTa   >  1:669625/1‑81 (MQ=255)                                                                        aCTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTa   <  2:669594/81‑1 (MQ=255)                                                                         cTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAt  <  2:669595/81‑1 (MQ=255)                                                                             |                                                                             TTAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAT  >  NC_000913/3740582‑3740734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑