Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,820,029	A→G	V381A (GTG→GCG)	ligB ←	DNA ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,820,029	0	A	G	100.0%	93.0 / NA	27	V381A (GTG→GCG)	ligB	DNA ligase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

AGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACT  >  NC_000913/3819953‑3820104                                                                             |                                                                            aGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGcgccacgcca                                                                          <  1:684796/80‑1 (MQ=255)    aGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCAcatc                                                                      <  1:684797/81‑1 (MQ=255)                  tCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGaa                                                        >  2:684833/1‑81 (MQ=255)                         gTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCt                                                  >  2:684842/1‑80 (MQ=255)                         gTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCt                                                  >  1:684825/1‑80 (MQ=255)                               gCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCgg                                            >  2:684852/1‑80 (MQ=255)                                 ggTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGc                                           >  2:684844/1‑79 (MQ=255)                                 ggTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCaa                                         <  1:684798/81‑1 (MQ=255)                                      tCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCt                                     <  2:684799/80‑1 (MQ=255)                                       cTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGa                                   >  2:684831/1‑81 (MQ=255)                                       cTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGa                                   >  1:684839/1‑81 (MQ=255)                                       cTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGa                                   >  1:684847/1‑81 (MQ=255)                                          gCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGAc                                  <  2:684801/79‑1 (MQ=255)                                          gCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGAc                                  <  2:684802/79‑1 (MQ=255)                                          gCGGTGTCGGTTTTGTACGTTCTGCACCACGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGAc                                  <  2:684800/79‑1 (MQ=255)                                           cGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACgag                               <  2:684803/81‑1 (MQ=255)                                            ggTGTCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACgag                               >  2:684830/1‑80 (MQ=255)                                             gtgtCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGaa                             >  2:684840/1‑81 (MQ=255)                                               gtCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATc                           >  2:684836/1‑81 (MQ=255)                                               gtCGGTTTTGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATc                           >  2:684851/1‑81 (MQ=255)                                                    ttttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATc                      <  1:684804/81‑1 (MQ=255)                                                     tttGTACGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACTCTGACCGGCAAGGCTGACGAGAATCTGATCa                     >  2:684846/1‑81 (MQ=255)                                                           cGTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAgg                <  2:684805/80‑1 (MQ=255)                                                            gTTCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcg              <  1:684806/81‑1 (MQ=255)                                                              tCTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGgcgc             >  1:684837/1‑80 (MQ=255)                                                               cTGCACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCa            <  1:684807/80‑1 (MQ=255)                                                                  cACCGCGCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAatat        <  1:684808/81‑1 (MQ=255)                                                                     cgcgCCACGCCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATcc      >  1:684845/1‑80 (MQ=255)                                                                        gccacgccacATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACt  >  2:684866/1‑81 (MQ=255)                                                                             |                                                                            AGAAGCAAAGTAGCAGGTCAACGAGTTAAAGCGGTTTTCTGGCGGTGTCGGTTTTGTACGTTCTGCACCGCGCCACACCACATCATCAATGCGAGGAATACCCTGACCGGCAAGGCTGACGAGAATCTGATCACCAGGCGCAATATCCCACT  >  NC_000913/3819953‑3820104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑