Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	414,600	C→T	W385* (TGG→TAG)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	414,600	0	C	T	100.0%	118.4 / NA	34	W385* (TGG→TAG)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/21);  total (13/21)

CTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTG  >  NC_000913/414523‑414676                                                                              |                                                                             cTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTAtt                                                                           <  1:80252/81‑1 (MQ=255)   tCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGc                                                                         >  2:80304/1‑81 (MQ=255)     aTCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCg                                                                       <  2:80253/81‑1 (MQ=255)      tCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGc                                                                      <  2:80254/81‑1 (MQ=255)           ggTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATg                                                                 >  1:80313/1‑81 (MQ=255)             ttAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCt                                                               <  2:80255/81‑1 (MQ=255)                aCGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTcgc                                                            <  2:80256/81‑1 (MQ=255)                  gTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGa                                                          >  1:80307/1‑81 (MQ=255)                     aaCGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATc                                                        <  2:80257/80‑1 (MQ=255)                           atagccgcAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGtt                                                 <  2:80259/78‑1 (MQ=255)                           aTCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGt                                                  <  2:80258/80‑1 (MQ=255)                            tCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGttt                                                >  1:80321/1‑81 (MQ=255)                                cgcAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTttgttg                                            >  2:80317/1‑81 (MQ=255)                                 gcAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTgg                                           >  2:80308/1‑81 (MQ=255)                                  cAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTggg                                          <  2:80260/81‑1 (MQ=255)                                   aaGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTgg                                           >  2:80328/1‑79 (MQ=255)                                       gcATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAAc                                     <  1:80261/81‑1 (MQ=255)                                       gcATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAAc                                     >  2:80316/1‑81 (MQ=255)                                        cATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACt                                    >  1:80333/1‑81 (MQ=255)                                          ttAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTgc                                  <  2:80262/81‑1 (MQ=255)                                             aGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCa                               <  1:80263/81‑1 (MQ=255)                                              gTTTTTGCTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCAc                              <  1:80264/81‑1 (MQ=255)                                                     cTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCTGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCTAcc                       >  1:80338/1‑81 (MQ=255)                                                     cTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAAcc                       <  1:80266/81‑1 (MQ=255)                                                     cTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAAcc                       <  2:80267/81‑1 (MQ=255)                                                     cTCAGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAAcc                       <  1:80265/81‑1 (MQ=255)                                                        aGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAAccc                      <  2:80269/79‑1 (MQ=255)                                                        aGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACccgc                    <  1:80270/81‑1 (MQ=255)                                                        aGCATGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACccg                     <  1:80268/80‑1 (MQ=255)                                                           aTGGGTTAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCgg                 >  2:80324/1‑81 (MQ=255)                                                                ttAACTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCgt            <  1:80271/81‑1 (MQ=255)                                                                  aaCTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCgttg          >  2:80315/1‑81 (MQ=255)                                                                   aCTGTTGCTGCTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCgttgt         >  2:80326/1‑81 (MQ=255)                                                                         gctgctATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACt   >  1:80322/1‑81 (MQ=255)                                                                          ctgcTATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTg  <  2:80272/81‑1 (MQ=255)                                                                              |                                                                             CTTCATCGGCGGTTAACGTCAACGTGATCGCCGCAAGCGCATTAAGTTTTTGCTCAGCATGGGTTAACTGTTGCTGCCATTGCCGCAGATGCTCGCGATCGCTGGTTTGTTGGGAGAACTGCGCACGCCAACCCGCCGGTTCGTTGTTCCACTG  >  NC_000913/414523‑414676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑