Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,834,019	C→T	V173V (GTG→GTA)	yicI ←	alpha‑D‑xyloside xylohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,834,019	0	C	T	100.0%	101.7 / NA	30	V173V (GTG→GTA)	yicI	alpha‑D‑xyloside xylohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

ACGGGATATTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGCACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATAAT  >  NC_000913/3833943‑3834095                                                                             |                                                                             aCGGGATATTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTAc                                                                            >  2:688076/1‑79 (MQ=255)             tataCGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAgc                                                              >  2:688095/1‑81 (MQ=255)              ataCGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAgc                                                              <  1:688044/80‑1 (MQ=255)                    cTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTcc                                                       <  2:688045/81‑1 (MQ=255)                       ttCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTccc                                                      <  1:688046/79‑1 (MQ=255)                         cAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAc                                                  >  1:688091/1‑81 (MQ=255)                         cAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAc                                                  >  1:688088/1‑81 (MQ=255)                          aGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAcc                                                 <  2:688047/81‑1 (MQ=255)                          aGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAc                                                  <  2:688048/80‑1 (MQ=255)                              cTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTa                                              >  1:688100/1‑80 (MQ=255)                                tgtgCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAAc                                           >  1:688107/1‑81 (MQ=255)                                  tgCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTg                                         >  2:688079/1‑81 (MQ=255)                                      gccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTc                                     >  1:688068/1‑81 (MQ=255)                                         gTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgc                                   >  2:688094/1‑80 (MQ=255)                                           cccGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAATGCGCTCTCCCAGACCGTAAACTGTTTcgccta                                >  2:688103/1‑79 (MQ=255)                                           cccGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgtcaa                                >  2:688102/1‑81 (MQ=255)                                              ggTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccgacgc                             <  2:688049/81‑1 (MQ=255)                                              ggTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgc                             <  1:688050/81‑1 (MQ=255)                                                ttCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgcca                           <  2:688051/81‑1 (MQ=255)                                                    aGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGAt                       <  2:688052/81‑1 (MQ=255)                                                     ggTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATc                      <  1:688054/81‑1 (MQ=255)                                                     ggTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATc                      <  1:688053/81‑1 (MQ=255)                                                      gTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATc                      >  2:688099/1‑80 (MQ=255)                                                             ccGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGc               <  2:688055/80‑1 (MQ=255)                                                             ccGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCt              <  1:688056/81‑1 (MQ=255)                                                              cGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCt              >  2:688093/1‑80 (MQ=255)                                                                    gCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACat       <  1:688058/81‑1 (MQ=255)                                                                    gCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACa        <  1:688057/80‑1 (MQ=255)                                                                       aTTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACAtata    <  1:688059/81‑1 (MQ=255)                                                                         tGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATAAt  >  2:688096/1‑81 (MQ=255)                                                                             |                                                                             ACGGGATATTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGCACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATAAT  >  NC_000913/3833943‑3834095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑