Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,907,030	C→A	S183S (TCG→TCT)	phoU ←	negative regulator of the pho regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,907,030	0	C	A	100.0%	130.2 / NA	37	S183S (TCG→TCT)	phoU	negative regulator of the pho regulon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/12);  total (25/12)

AATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGA  >  NC_000913/3906952‑3907105                                                                               |                                                                            aaTATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACgaga                                                                           <  2:703062/81‑1 (MQ=255)     ttCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGTAGTAAGTACGCTCGGAATGGTACGAGAATCt                                                                       >  2:703098/1‑81 (MQ=255)     ttCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCt                                                                       <  1:703063/81‑1 (MQ=255)       cTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCtt                                                                      <  1:703064/80‑1 (MQ=255)           cAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCcat                                                                  >  2:703085/1‑80 (MQ=255)             gCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCcatcat                                                               <  2:703065/81‑1 (MQ=255)              cGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATg                                                              <  1:703066/81‑1 (MQ=255)               ggTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATg                                                              >  2:703067/1‑80 (MQ=255)                    ccAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGt                                                         >  1:703092/1‑80 (MQ=255)                    ccAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGt                                                         >  1:703094/1‑80 (MQ=255)                        tACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGtcat                                                      >  1:703116/1‑79 (MQ=255)                          cGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAg                                                   <  1:703068/80‑1 (MQ=255)                           gTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGtt                                                 >  1:703096/1‑81 (MQ=255)                                aTAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACg                                             >  1:703100/1‑80 (MQ=255)                                     aCGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACaa                                        >  2:703107/1‑80 (MQ=255)                                      cgcgcgCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATa                                      >  1:703101/1‑81 (MQ=255)                                      cgcgcgCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATa                                      >  1:703117/1‑81 (MQ=255)                                       gcgcgcAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATAc                                     <  1:703069/81‑1 (MQ=255)                                           gcAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTc                                 >  1:703090/1‑81 (MQ=255)                                             aGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCg                                >  2:703115/1‑80 (MQ=255)                                             aGAACAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCg                                >  1:703108/1‑80 (MQ=255)                                                 cAGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTAGTc                           >  2:703099/1‑81 (MQ=255)                                                  aGCGCAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTcc                          <  1:703070/81‑1 (MQ=255)                                                     gcAGTAAGTACGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGa                       >  1:703103/1‑81 (MQ=255)                                                     gaagtaagtaCGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGa                       >  2:703102/3‑81 (MQ=255)                                                           agtaCGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGAc                  <  2:703071/80‑1 (MQ=255)                                                            gtaCGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACtt                >  2:703110/1‑81 (MQ=255)                                                             taCGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttt               <  1:703072/81‑1 (MQ=255)                                                               cGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttttt             >  2:703112/1‑81 (MQ=255)                                                               cGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttttt             <  2:703073/81‑1 (MQ=255)                                                               cGCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttttt             >  2:703074/1‑81 (MQ=255)                                                                gCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACtttttt            <  1:703075/81‑1 (MQ=255)                                                                gCTCGGAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACttttt             >  1:703119/1‑80 (MQ=255)                                                                    ggAATGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCt        >  2:703104/1‑81 (MQ=255)                                                                      aaTGGTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTc      <  2:703076/81‑1 (MQ=255)                                                                          gTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGa  >  1:703106/1‑81 (MQ=255)                                                                          gTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGa  >  1:703111/1‑81 (MQ=255)                                                                          gTACGAGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGa  >  1:703095/1‑81 (MQ=255)                                                                               |                                                                            AATATTCTGGCAGCGGTCGCCAATACGTTCGATAGAACGCGCGCAGAACAGCGCAGTAAGTACGCTCGGAATGGTACGCGAATCTTCCATCATGTAGGTCATCAGTTGACGAACAATACCTTCGTATTCCTGATCGACTTTTTTATCTTCACGA  >  NC_000913/3906952‑3907105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑