Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,910,137	T→A	I88F (ATC→TTC)	pstC ←	phosphate ABC transporter membrane subunit PstC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,910,137	0	T	A	100.0%	94.9 / NA	28	I88F (ATC→TTC)	pstC	phosphate ABC transporter membrane subunit PstC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (8/20);  total (8/20)

GGGCGTTTCAGCCAGCCAGGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGATCAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCGGTGCATC  >  NC_000913/3910067‑3910212                                                                       |                                                                            gggCGTTTCAGCCAGCCAGGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAgcgc                                                                    >  1:703904/1‑80 (MQ=255) gggCGTTTCAGCCAGCCAGGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGcgcg                                                                   <  1:703852/81‑1 (MQ=255)   gCGTTTCAGCCAGCCAGGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGAt                                                                 <  1:703854/81‑1 (MQ=255)   gCGTTTCAGCCAGCCAGGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGAt                                                                 <  2:703853/81‑1 (MQ=255)         cagccagcCAGGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACg                                                            >  2:703905/1‑80 (MQ=255)          agccagccAGGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGa                                                           <  1:703855/80‑1 (MQ=255)                  agGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCaa                                                  <  1:703856/81‑1 (MQ=255)                   ggCGCAAACTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAAc                                                 <  1:703857/81‑1 (MQ=255)                      gcAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTa                                              >  2:703892/1‑81 (MQ=255)                       cAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTAc                                             <  1:703859/81‑1 (MQ=255)                       cAAACTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTAc                                             <  2:703858/81‑1 (MQ=255)                        aaGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTAcc                                            <  2:703860/81‑1 (MQ=255)                          gCTCAGACAGGAACAGTGCGATACCGAAACTCCCAGGGACGGCGTACAGCAGCGCGATAAGCGGAGTCGCCAACGTACCGt                                          <  2:703861/81‑1 (MQ=255)                                  agGAACAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGc                                  <  2:703862/81‑1 (MQ=255)                                     aaCAGGGCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTATCGTAGATCGGCAcc                               <  1:703863/81‑1 (MQ=255)                                         gggCGATACCGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAgcg                           <  1:703864/81‑1 (MQ=255)                                             gATACCGAAACTCACCGGGACGGCGAACAGCGGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGcccc                       <  2:703865/81‑1 (MQ=255)                                                 ccGAAACTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAg                   <  1:703866/81‑1 (MQ=255)                                                      aCTCACCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATc              <  2:703867/81‑1 (MQ=255)                                                          aCCGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTc          <  1:703868/81‑1 (MQ=255)                                                           ccGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCg         >  1:703926/1‑81 (MQ=255)                                                            cGGGACGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCgg        >  1:703899/1‑81 (MQ=255)                                                              ggACGGCGAACAGCAGCGCGATAAGCGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCGGTg      >  2:703900/1‑81 (MQ=255)                                                                aCGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCGGTGCa    >  2:703901/1‑81 (MQ=255)                                                                 cGGCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCGGTGCAt   <  2:703869/81‑1 (MQ=255)                                                                  ggCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCGGTGCATc  <  1:703871/81‑1 (MQ=255)                                                                  ggCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCGGTGCATc  >  1:703903/1‑81 (MQ=255)                                                                  ggCGAACAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCGGTGCATc  <  2:703870/81‑1 (MQ=255)                                                                       |                                                                            GGGCGTTTCAGCCAGCCAGGCGCAAGCTCAGTCAGGAACAGGGCGATACCGAAACTCACCGGGACGGCGATCAGCAGCGCGATAAACGAAGTCACCAACGTACCGTAGATCGGCACCAGCGCCCCGTAGATATCGTTCGGTGCATC  >  NC_000913/3910067‑3910212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑