Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,924,204	C→T	R477H (CGT→CAT)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,924,204	0	C	T	100.0%	116.5 / NA	33	R477H (CGT→CAT)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

CGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCA  >  NC_000913/3924128‑3924275                                                                             |                                                                        cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAgacgctgatgct                                                                      >  1:707116/1‑80 (MQ=255) cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAgacgctgatgct                                                                      >  1:707107/1‑80 (MQ=255) cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGatgctc                                                                     <  2:707050/81‑1 (MQ=255) cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGatgctc                                                                     <  2:707049/81‑1 (MQ=255)        gTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCg                                                              <  2:707051/81‑1 (MQ=255)          cAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGa                                                             >  2:707125/1‑80 (MQ=255)           aGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGat                                                            <  2:707052/80‑1 (MQ=255)            ggTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGatat                                                          <  1:707053/81‑1 (MQ=255)                  cATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCaa                                                    <  1:707054/81‑1 (MQ=255)                   aTTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAg                                                   <  1:707055/81‑1 (MQ=255)                     tCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGtt                                                 >  1:707102/1‑81 (MQ=255)                           cGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTtctc                                             <  1:707056/79‑1 (MQ=255)                            ggCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGtt                                          <  2:707057/81‑1 (MQ=255)                             gCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGtt                                          >  1:707113/1‑80 (MQ=255)                             gCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTa                                         <  2:707058/81‑1 (MQ=255)                              cTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTa                                         >  1:707112/1‑80 (MQ=255)                                gCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAg                                      >  2:707108/1‑81 (MQ=255)                                  aGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcg                                    >  2:707115/1‑81 (MQ=255)                                    cTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgcg                                  <  1:707059/81‑1 (MQ=255)                                         gccgACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCaa                             >  1:707120/1‑81 (MQ=255)                                            gACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGt                          >  2:707119/1‑81 (MQ=255)                                              cGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTc                        <  2:707060/81‑1 (MQ=255)                                              cGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTc                        <  2:707061/81‑1 (MQ=255)                                               ggggTTACCCAGGTCGATTTCAGACGCTGGTGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCg                       >  1:707111/1‑81 (MQ=255)                                               ggggTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCg                       >  1:707127/1‑81 (MQ=255)                                                   ttACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt                   <  1:707063/81‑1 (MQ=255)                                                   ttACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt                   <  1:707062/81‑1 (MQ=255)                                                   ttACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt                   <  2:707064/81‑1 (MQ=255)                                                     aCCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATc                  <  1:707065/80‑1 (MQ=255)                                                      cccAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcca                >  1:707123/1‑81 (MQ=255)                                                       ccAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccac               >  2:707118/1‑81 (MQ=255)                                                        cAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccac               >  2:707114/1‑80 (MQ=255)                                                            tCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGc          >  2:707122/1‑81 (MQ=255)                                                                tttCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAg      >  1:707121/1‑81 (MQ=255)                                                                   cAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGtt    <  2:707066/80‑1 (MQ=255)                                                                    aGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCa  <  1:707067/81‑1 (MQ=255)                                                                    aGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCa  >  1:707117/1‑81 (MQ=255)                                                                             |                                                                        CGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCA  >  NC_000913/3924128‑3924275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑