Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	415,362	T→C	D131G (GAT→GGT)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	415,362	0	T	C	100.0%	109.2 / NA	31	D131G (GAT→GGT)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/11);  total (20/11)

GCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTATCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTACCGTC  >  NC_000913/415285‑415438                                                                              |                                                                             gCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCt                                                                            <  2:80437/80‑1 (MQ=255)   cccTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCttt                                                                          >  1:80471/1‑80 (MQ=255)        cGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACttt                                                                    <  2:80438/81‑1 (MQ=255)         gAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTg                                                                   >  1:80461/1‑81 (MQ=255)              cagcaTCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGc                                                              <  2:80439/81‑1 (MQ=255)                  aTCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCgag                                                           >  2:80466/1‑80 (MQ=255)                  aTCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCgag                                                           >  2:80462/1‑80 (MQ=255)                     gAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAAtt                                                       >  2:80474/1‑81 (MQ=255)                         gggTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGc                                                   >  1:80472/1‑81 (MQ=255)                           gTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCg                                                 >  2:80460/1‑81 (MQ=255)                                gcgcCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGgc                                            >  1:80465/1‑81 (MQ=255)                                 cgcCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGgcg                                           <  1:80440/81‑1 (MQ=255)                                  gcCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGgcg                                           >  2:80477/1‑80 (MQ=255)                                  gcCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGgcg                                           >  1:80479/1‑80 (MQ=255)                                  gcCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGgcg                                           >  1:80486/1‑80 (MQ=255)                                   cccGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCa                                         <  2:80441/81‑1 (MQ=255)                                     cGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAgc                                       <  2:80442/81‑1 (MQ=255)                                      gTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAgcg                                      >  1:80470/1‑81 (MQ=255)                                         aTCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAgcgcgc                                   >  1:80488/1‑81 (MQ=255)                                              gCCCGGTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGct                              <  2:80443/81‑1 (MQ=255)                                                  ggTTAACGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTAc                          <  1:80444/81‑1 (MQ=255)                                                      aaCGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGt                      >  2:80495/1‑81 (MQ=255)                                                       aCGTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGt                      <  2:80445/80‑1 (MQ=255)                                                         gTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTgg                    >  1:80496/1‑80 (MQ=255)                                                         gTCGCTGTCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTgg                    >  2:80487/1‑80 (MQ=255)                                                                tCAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTg              <  1:80446/79‑1 (MQ=255)                                                                 cAGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCa            >  1:80469/1‑80 (MQ=255)                                                                  aGTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCaaa          >  2:80478/1‑81 (MQ=255)                                                                   gTTCCAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAAt         >  1:80514/1‑81 (MQ=255)                                                                       cAGCTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTAcc     >  1:80498/1‑81 (MQ=255)                                                                          cTTACCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTACCGTc  <  1:80447/81‑1 (MQ=255)                                                                              |                                                                             GCCCCTGCGAAAGCAGCATCGAACGGGTGAAGCGCCCGTAATCCAGCCCGGTTAACGTCGCTGTCAGTTCCAGCTTATCTTTCACTTTGTCGGCGAGAATTTTGCCGTCGGCGCAGCGCGCCAGCTCTACGCGTGGCACCTGCAAATTACCGTC  >  NC_000913/415285‑415438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑