Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,940,461	G→A	A51V (GCC→GTC)	hsrA ←	putative transporter HsrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,940,461	0	G	A	100.0%	140.7 / NA	41	A51V (GCC→GTC)	hsrA	putative transporter HsrA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

CGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCA  >  NC_000913/3940383‑3940538                                                                               |                                                                              cGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACt                                                                             <  1:710587/81‑1 (MQ=255)  gACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACt                                                                             >  1:710643/1‑80 (MQ=255)   aCGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGa                                                                           >  2:710651/1‑81 (MQ=255)    cgcgTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGAt                                                                          <  2:710588/81‑1 (MQ=255)       gTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGc                                                                       <  1:710589/81‑1 (MQ=255)            gAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATcgc                                                                  <  2:710590/81‑1 (MQ=255)             aaGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATcgcg                                                                 <  2:710591/81‑1 (MQ=255)                 gATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGagag                                                             >  1:710633/1‑81 (MQ=255)                  aTCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAgagg                                                            <  1:710592/81‑1 (MQ=255)                     ggCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAgaggag                                                          >  2:710634/1‑80 (MQ=255)                     ggCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAgaggag                                                          >  1:710638/1‑80 (MQ=255)                            cATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGAtt                                                   >  2:710637/1‑80 (MQ=255)                                cGCTTACCGGAATAAGCATCGCCACCGTCAGCGTTTAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGc                                              <  2:710593/81‑1 (MQ=255)                                 gCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGc                                              >  2:710647/1‑80 (MQ=255)                                 gCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCt                                             <  2:710594/81‑1 (MQ=255)                                    tACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTAt                                           >  1:710595/1‑80 (MQ=255)                                     aCCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTAt                                           <  2:710596/79‑1 (MQ=255)                                         gAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGa                                     <  1:710597/81‑1 (MQ=255)                                             aaGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGc                                 >  2:710644/1‑81 (MQ=255)                                              aGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCg                                <  2:710598/81‑1 (MQ=255)                                                 aTCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGt                             <  1:710599/81‑1 (MQ=255)                                                   cGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGt                             >  1:710654/1‑79 (MQ=255)                                                   cGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGt                             >  2:710639/1‑79 (MQ=255)                                                    gCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTaa                           <  2:710600/80‑1 (MQ=255)                                                      cACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGc                        >  1:710671/1‑81 (MQ=255)                                                      cACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGc                        >  2:710648/1‑81 (MQ=255)                                                         cGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCgg                      >  2:710646/1‑80 (MQ=255)                                                         cGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGt                     <  1:710601/81‑1 (MQ=255)                                                          gTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTa                    >  2:710649/1‑81 (MQ=255)                                                          gTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTa                    >  2:710658/1‑81 (MQ=255)                                                           tCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTAt                   >  1:710656/1‑81 (MQ=255)                                                            cAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTAt                   >  2:710660/1‑80 (MQ=255)                                                             aGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTAtt                  <  1:710602/80‑1 (MQ=255)                                                                gTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAg               >  2:710657/1‑80 (MQ=255)                                                                gTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGa              <  1:710603/81‑1 (MQ=255)                                                                gTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGa              <  1:710604/81‑1 (MQ=255)                                                                   taACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAAt            <  2:710605/80‑1 (MQ=255)                                                                      cTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTg        <  1:710606/81‑1 (MQ=255)                                                                       tgatgatgaCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTgg       >  2:710673/1‑81 (MQ=255)                                                                       tgatgatgaCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTgg       >  2:710682/1‑81 (MQ=255)                                                                        gatgatGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTgg       >  2:710655/1‑80 (MQ=255)                                                                         atgatgACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGc      <  2:710607/80‑1 (MQ=255)                                                                            atgACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCa  <  1:710608/81‑1 (MQ=255)                                                                               |                                                                              CGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCA  >  NC_000913/3940383‑3940538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑