Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,033,383	T→A	L80Q (CTG→CAG)	trkH →	K(+) transporter TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,033,383	0	T	A	100.0%	107.3 / NA	31	L80Q (CTG→CAG)	trkH	K(+) transporter TrkH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/14);  total (17/14)

GGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTT  >  NC_000913/4033305‑4033453                                                                               |                                                                       ggtggCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAgg                                                                      >  1:731193/1‑81 (MQ=255)         aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc                                                              >  2:731202/1‑81 (MQ=255)         aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc                                                              >  1:731197/1‑81 (MQ=255)          aCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCg                                                             >  2:731205/1‑81 (MQ=255)           ccGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCgg                                                            <  1:731157/81‑1 (MQ=255)                aaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTgcgc                                                       <  2:731158/81‑1 (MQ=255)                aaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGT‑GTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCt                                                      >  1:731201/1‑81 (MQ=255)                 agagAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCt                                                      >  1:731203/1‑81 (MQ=255)                    gaAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTccc                                                   >  2:731208/1‑81 (MQ=255)                    gaAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTccc                                                   <  2:731160/81‑1 (MQ=255)                    gaAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTccc                                                   <  2:731159/81‑1 (MQ=255)                       aGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCttt                                                >  1:731220/1‑81 (MQ=255)                        ggCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCtttt                                               <  2:731161/81‑1 (MQ=255)                             aCTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTAtctt                                          <  1:731162/81‑1 (MQ=255)                                tAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTtctc                                       >  1:731206/1‑81 (MQ=255)                                 aaaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTAtcttct                                        <  1:731163/79‑1 (MQ=255)                                 aaaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTtctc                                       <  2:731164/80‑1 (MQ=255)                                  aaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCg                                      >  2:731200/1‑80 (MQ=255)                                     cccGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGa                                    >  2:731199/1‑79 (MQ=255)                                        gTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGcc                               >  2:731227/1‑81 (MQ=255)                                           aGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCg                             >  2:731211/1‑80 (MQ=255)                                             gggTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAAc                          <  1:731165/81‑1 (MQ=255)                                                 ttCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCa                      >  1:731218/1‑81 (MQ=255)                                                   cTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCAc                     <  2:731166/80‑1 (MQ=255)                                                         gtggtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTAcc              <  1:731167/81‑1 (MQ=255)                                                            gtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGa            <  2:731169/80‑1 (MQ=255)                                                            gtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGAt           <  2:731168/81‑1 (MQ=255)                                                                  ttCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGttt     <  2:731170/81‑1 (MQ=255)                                                                   tCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtttt    >  2:731229/1‑81 (MQ=255)                                                                      ggACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtttttt  >  2:731222/1‑80 (MQ=255)                                                                      ggACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtttttt  >  1:731249/1‑80 (MQ=255)                                                                               |                                                                       GGTGGCCGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTT  >  NC_000913/4033305‑4033453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑