Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,081,536	A→C	F109V (TTC→GTC)	fdoI ←	formate dehydrogenase O subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,081,536	0	A	C	100.0%	79.7 / NA	23	F109V (TTC→GTC)	fdoI	formate dehydrogenase O subunit gamma
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/14);  total (9/14)

CGCCAGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGAAGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCAT  >  NC_000913/4081460‑4081610                                                                             |                                                                           cGCCAGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTta                                                                        <  2:740845/81‑1 (MQ=255)   ccAGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTtat                                                                       >  1:740873/1‑80 (MQ=255)          aTCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGccc                                                                <  2:740846/80‑1 (MQ=255)             aCGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGgtg                                                            <  1:740847/81‑1 (MQ=255)                        cagcagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTc                                                 <  2:740848/81‑1 (MQ=255)                        cagcagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTc                                                 <  2:740849/81‑1 (MQ=255)                          gcagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCt                                               >  2:740871/1‑81 (MQ=255)                           cagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTc                                              >  2:740872/1‑81 (MQ=255)                           cagcagAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTc                                              <  2:740850/81‑1 (MQ=255)                               aggaCCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTg                                          <  2:740851/78‑1 (MQ=255)                               agAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGtt                                           >  1:740878/1‑80 (MQ=255)                                     aGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTgacgac                                     >  2:740883/1‑80 (MQ=255)                                       gAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGAt                                  <  2:740852/81‑1 (MQ=255)                                            taatCGCCGCCCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTAc                             <  1:740853/81‑1 (MQ=255)                                                 gccgccCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAat                         <  1:740854/80‑1 (MQ=255)                                                 gcagccCAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAat                         <  2:740855/77‑1 (MQ=255)                                                       cAGAAAACGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATAttcttc                  <  2:740856/81‑1 (MQ=255)                                                            aaCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCa             <  1:740857/81‑1 (MQ=255)                                                             aCGCATTTCTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCaa            <  2:740858/81‑1 (MQ=255)                                                                     cTGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTGGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGatat     >  2:740876/1‑80 (MQ=255)                                                                      tGACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATatca   >  2:740901/1‑81 (MQ=255)                                                                       gACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATatcat  >  1:740885/1‑81 (MQ=255)                                                                       gACCGACGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATatcat  >  2:740881/1‑81 (MQ=255)                                                                             |                                                                           CGCCAGATAATCACGCCGCTCACCAGCAGCAGAACCAGGAAAATAATCGCCGCCCAGAAAACGCATTTCTGACCGAAGTTATAACGCCCGGTGTCACCTACTTCCTCGTTGACGACGATCTTACGAATATTCTTCGCCCAAAAGATATCAT  >  NC_000913/4081460‑4081610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑