Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	428,924	C→T	A426A (GCC→GCT)	secD →	Sec translocon accessory complex subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	428,924	0	C	T	100.0%	141.1 / NA	40	A426A (GCC→GCT)	secD	Sec translocon accessory complex subunit SecD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/24);  total (16/24)

CGTCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCCGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGCAGAACATTGAAC  >  NC_000913/428847‑429000                                                                              |                                                                             cGTCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGt                                                                           >  2:83171/1‑81 (MQ=255)   tCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGc                                                                         >  2:83161/1‑81 (MQ=255)   tCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGc                                                                         <  2:83114/81‑1 (MQ=255)   tCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGc                                                                         <  2:83113/81‑1 (MQ=255)   tCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGc                                                                         <  2:83112/81‑1 (MQ=255)     tGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGt                                                                       >  2:83169/1‑81 (MQ=255)       ggTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGtt                                                                      <  1:83115/80‑1 (MQ=255)          aaCAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATc                                                                  >  1:83179/1‑81 (MQ=255)           aCAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATc                                                                  <  2:83116/80‑1 (MQ=255)            cAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATcgc                                                                <  2:83117/81‑1 (MQ=255)               cTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGcc                                                             <  1:83118/81‑1 (MQ=255)               cTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGcc                                                             >  2:83164/1‑81 (MQ=255)               cTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGcc                                                             <  1:83119/81‑1 (MQ=255)                    gTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTc                                                        <  2:83121/81‑1 (MQ=255)                    gTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTc                                                        <  2:83120/81‑1 (MQ=255)                      aTCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGAtt                                                         >  1:83160/1‑78 (MQ=255)                          ccGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGAttg                                                  <  1:83122/81‑1 (MQ=255)                           cGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGAttgt                                                 <  1:83123/81‑1 (MQ=255)                                tCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTgaag                                            <  2:83124/81‑1 (MQ=255)                                   acaacCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAAc                                         >  1:83168/1‑81 (MQ=255)                                     aacCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGt                                       <  2:83125/81‑1 (MQ=255)                                          gaacgaaGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCAt                                  >  2:83172/1‑81 (MQ=255)                                          gaacgaaGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCAt                                  >  2:83166/1‑81 (MQ=255)                                            acgaaGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTg                                >  1:83186/1‑81 (MQ=255)                                              gaaGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGc                              >  1:83185/1‑81 (MQ=255)                                                aGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGccc                            <  2:83127/81‑1 (MQ=255)                                                aGCCCGTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGccc                            <  1:83126/81‑1 (MQ=255)                                                     gTCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAAccc                       >  1:83162/1‑81 (MQ=255)                                                      tCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCt                      <  1:83128/81‑1 (MQ=255)                                                      tCAGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCt                      <  2:83129/81‑1 (MQ=255)                                                        aGCTGTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTgg                    >  2:83170/1‑81 (MQ=255)                                                            gTCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTAt                >  2:83175/1‑81 (MQ=255)                                                             tCACTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTa                 <  2:83130/79‑1 (MQ=255)                                                               aCTGCTGCTGCGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGc              <  1:83131/80‑1 (MQ=255)                                                                      ctgcGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGCAGAACAtt      <  2:83132/81‑1 (MQ=255)                                                                      ctgcGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGCAGAACAtt      >  1:83165/1‑81 (MQ=255)                                                                         cGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGCAGAACATTg     <  2:83134/79‑1 (MQ=255)                                                                         cGTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGCAGAACATTGa    <  2:83133/80‑1 (MQ=255)                                                                          gTGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGCAGAACATTGAAc  <  1:83135/81‑1 (MQ=255)                                                                           tGCTGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGCAGAACATTGaa   >  1:83177/1‑79 (MQ=255)                                                                              |                                                                             CGTCTGGGTAACAGCTTCCGTATCACCGGCATCAACAACCCGAACGAAGCCCGTCAGCTGTCACTGCTGCTGCGTGCCGGTGCGTTGATCGCGCCGATTCAGATTGTTGAAGAACGTACCATTGGCCCAACCCTGGGTATGCAGAACATTGAAC  >  NC_000913/428847‑429000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑