Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,104,429	C→T	A183T (GCC→ACC)	cpxA ←	sensory histidine kinase CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,104,429	0	C	T	100.0%	104.2 / NA	30	A183T (GCC→ACC)	cpxA	sensory histidine kinase CpxA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/16);  total (14/16)

TGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTT  >  NC_000913/4104353‑4104504                                                                             |                                                                            tGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAAc                                                                         <  1:745857/81‑1 (MQ=255) tGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAAc                                                                         <  2:745855/81‑1 (MQ=255) tGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAAc                                                                         <  1:745856/81‑1 (MQ=255)     ggCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACcac                                                                      >  2:745897/1‑80 (MQ=255)          aaGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAAcag                                                                 >  2:745894/1‑80 (MQ=255)           aGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAAcagc                                                                <  1:745858/80‑1 (MQ=255)               tCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcgg                                                           >  1:745901/1‑81 (MQ=255)               tCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcg                                                            <  2:745859/80‑1 (MQ=255)                cccTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAgcgg                                                           <  2:745860/80‑1 (MQ=255)                  cTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGt                                                        <  2:745861/81‑1 (MQ=255)                    gggCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTAc                                                      >  2:745882/1‑81 (MQ=255)                           ttCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCaa                                               >  2:745895/1‑81 (MQ=255)                                 ggCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt                                         >  2:745902/1‑81 (MQ=255)                                  gCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt                                         >  2:745896/1‑80 (MQ=255)                                  gCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGt                                         >  1:745887/1‑80 (MQ=255)                                   cAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGa                                       <  2:745862/81‑1 (MQ=255)                                     gCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACa                                     <  2:745863/81‑1 (MQ=255)                                        tttttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAAt                                   >  2:745891/1‑80 (MQ=255)                                          tttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcag                                >  1:745904/1‑81 (MQ=255)                                           ttCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcag                                <  2:745864/80‑1 (MQ=255)                                            tCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagc                               <  2:745865/80‑1 (MQ=255)                                              aGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagcag                             <  2:745866/80‑1 (MQ=255)                                              aGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATcagcag                             >  1:745900/1‑80 (MQ=255)                                              aGCTTACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGt                            <  1:745867/81‑1 (MQ=255)                                                 ttACGCGCCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTaa                          >  2:745889/1‑80 (MQ=255)                                                      cgcCGGTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcgg                    <  2:745868/81‑1 (MQ=255)                                                          ggTTTTGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAgcgggcgg                <  1:745869/81‑1 (MQ=255)                                                              ttGCCAGACTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCa             >  1:745899/1‑80 (MQ=255)                                                                     aCTCCAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGt       >  2:745910/1‑79 (MQ=255)                                                                         cAGGTCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGtt  <  1:745870/80‑1 (MQ=255)                                                                             |                                                                            TGCTGGCGTAAGTTTCCCTGGGCAACTTCATCGGCAGCGTTTTTCAGCTTACGCGCCGGTTTTGCCAGACTCCAGGCCAACCACAACAGCAGCGGCGTACTGACCAACATGGTGACAATCAGCAGTAATAGCGGGCGGTCAAACAGTAAGTT  >  NC_000913/4104353‑4104504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑