Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,111,676	G→A	T180I (ACC→ATC)	yiiQ ←	DUF1454 domain‑containing protein YiiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,111,676	0	G	A	100.0%	88.3 / NA	26	T180I (ACC→ATC)	yiiQ	DUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/13);  total (13/13)

GCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT  >  NC_000913/4111602‑4111752                                                                           |                                                                             gctttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGcc                                                                        <  1:747399/81‑1 (MQ=255)              tCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGt                                                            <  1:747400/80‑1 (MQ=255)               cATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTg                                                          >  1:747436/1‑81 (MQ=255)                 tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTc                                                        <  1:747401/81‑1 (MQ=255)                     ttCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCcgc                                                     >  2:747427/1‑80 (MQ=255)                        aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGac                                                  <  1:747402/80‑1 (MQ=255)                        aaaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGac                                                  <  2:747403/80‑1 (MQ=255)                         aaCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGacaa                                                >  1:747426/1‑81 (MQ=255)                               cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATaa                                           <  1:747404/80‑1 (MQ=255)                               cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATa                                            <  2:747405/79‑1 (MQ=255)                               cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAAc                                          >  1:747439/1‑81 (MQ=255)                                aaGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACg                                         >  1:747423/1‑81 (MQ=255)                                aaGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACg                                         <  1:747406/81‑1 (MQ=255)                                    gATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGt                                     >  1:747424/1‑81 (MQ=255)                                              gcgcCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCt                            <  2:747407/80‑1 (MQ=255)                                              gcgcCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTg                           >  1:747437/1‑81 (MQ=255)                                                gcCAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtc                         >  1:747418/1‑81 (MQ=255)                                                 ccAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtct                        <  2:747408/81‑1 (MQ=255)                                                 ccAGCTTAATCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtc                         >  2:747428/1‑80 (MQ=255)                                                        aaTCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTAGgtgt                  <  2:747409/80‑1 (MQ=255)                                                          tCGGTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTAGGTGtaat               <  2:747410/81‑1 (MQ=255)                                                            ggTTCAACAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTAGGTGtaata              <  2:747411/80‑1 (MQ=255)                                                                 aaCAGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTAGGTGTAATAACgttt        >  2:747441/1‑81 (MQ=255)                                                                    aGCGAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTAGGTGTAATAACgtttgtt     >  1:747438/1‑81 (MQ=255)                                                                      cGAAGATCAGCCCCTTTTCGCCATTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTAGGTGTAATAACGTTTGtttt   >  2:747433/1‑81 (MQ=255)                                                                       gAAGATCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTAGGTGTAATAACGTTTGttttt  >  1:747432/1‑81 (MQ=255)                                                                           |                                                                             GCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT  >  NC_000913/4111602‑4111752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑