Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,126,469	C→A	P114P (CCG→CCT)	priA ←	primosome factor N'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,126,469	0	C	A	100.0%	171.8 / NA	49	P114P (CCG→CCT)	priA	primosome factor N'
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/25);  total (24/25)

CTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATCGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATCGGATGATG  >  NC_000913/4126391‑4126546                                                                               |                                                                              cTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAgg                                                                             >  1:750564/1‑81 (MQ=255)   gttgttGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGgcg                                                                           >  1:750552/1‑81 (MQ=255)    ttgttgCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGgcgc                                                                          >  1:750553/1‑81 (MQ=255)    ttgttgCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGgc                                                                            <  2:750493/79‑1 (MQ=255)    ttgttgCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGgc                                                                            <  1:750492/79‑1 (MQ=255)     tgttgCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGcgcg                                                                         >  2:750555/1‑81 (MQ=255)      gttgCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTTAGTGGCAAACCAGTACCACTTAGGCGCGt                                                                        >  2:750559/1‑81 (MQ=255)         gCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTc                                                                      <  1:750494/80‑1 (MQ=255)         gCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTc                                                                      <  2:750496/80‑1 (MQ=255)         gCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTc                                                                      <  2:750495/80‑1 (MQ=255)          cTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTcgc                                                                    <  1:750497/81‑1 (MQ=255)            tGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTcgccg                                                                  >  1:750549/1‑81 (MQ=255)            tGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTcgccg                                                                  >  2:750570/1‑81 (MQ=255)             gggggAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTcgccg                                                                  >  2:750565/1‑80 (MQ=255)                ggAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAgg                                                              <  2:750498/81‑1 (MQ=255)                     gTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGgccgcc                                                         >  1:750539/1‑81 (MQ=255)                     gTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGgccgcc                                                         <  2:750499/81‑1 (MQ=255)                      tttCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGccc                                                        <  1:750500/81‑1 (MQ=255)                         cAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCt                                                      <  1:750501/80‑1 (MQ=255)                         cAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTg                                                     >  1:750556/1‑81 (MQ=255)                          aGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTg                                                     <  1:750502/80‑1 (MQ=255)                          aGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTg                                                     <  2:750503/80‑1 (MQ=255)                           ggCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGc                                                   >  2:750568/1‑81 (MQ=255)                            gCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCg                                                  <  1:750505/81‑1 (MQ=255)                            gCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCg                                                  <  1:750504/81‑1 (MQ=255)                             cTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCgt                                                 <  2:750506/81‑1 (MQ=255)                                ttCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCgtag                                               <  2:750507/80‑1 (MQ=255)                                   aGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTaa                                            >  2:750560/1‑80 (MQ=255)                                   aGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTaa                                            >  1:750554/1‑80 (MQ=255)                                     aTCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAAt                                         >  1:750581/1‑81 (MQ=255)                                        cACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCgg                                      >  2:750579/1‑81 (MQ=255)                                        cACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCgg                                      <  1:750509/81‑1 (MQ=255)                                        cACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCg                                       <  1:750508/80‑1 (MQ=255)                                            gCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAg                                  >  1:750531/1‑81 (MQ=255)                                                     tGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGaaa                          >  1:750582/1‑80 (MQ=255)                                                     tGTTCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGaaa                          >  1:750572/1‑80 (MQ=255)                                                       ttCAGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAg                       >  1:750563/1‑81 (MQ=255)                                                          aGTGGCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGcac                    <  2:750510/81‑1 (MQ=255)                                                              gCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATc                 <  1:750512/80‑1 (MQ=255)                                                              gCAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCg                <  2:750511/81‑1 (MQ=255)                                                               cAAACCAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGc               <  2:750513/81‑1 (MQ=255)                                                                   ccAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAAt           >  2:750583/1‑81 (MQ=255)                                                                    cAGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATc          >  2:750585/1‑81 (MQ=255)                                                                     aGTACCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATCg         <  1:750514/81‑1 (MQ=255)                                                                        aCCACATAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATCGgat      >  2:750575/1‑81 (MQ=255)                                                                           acaTAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATCGgatgat   >  1:750591/1‑81 (MQ=255)                                                                           acaTAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATCGgatgat   >  2:750566/1‑81 (MQ=255)                                                                           acaTAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATCGgatgat   <  1:750515/81‑1 (MQ=255)                                                                            caTAGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATCGgatgatg  <  2:750516/81‑1 (MQ=255)                                                                               |                                                                              CTGTTGTTGCTTGGGGGAGCGTTTCAGGCTGTTCAGATCCACCGCCTGGCCTTGTTCAGTGGCAAACCAGTACCACATCGGCGCGTTCGCCGCAGGCCGCCCCTGGCGTAGTAAAATCGGCAAGGCATGAAACAGCACATCGCCAATCGGATGATG  >  NC_000913/4126391‑4126546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑