BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,142,361	G→T	intergenic (‑140/‑169)	ptsA ← / → frwC	putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,142,361	0	G	T	100.0%	83.9 / NA	25	intergenic (‑140/‑169)	ptsA/frwC	putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (11/14); total (11/14)

TGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGG  >  NC_000913/4142283‑4142436

tgtgACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTtgctgc                                                                           <  2:754061/81‑1 (MQ=255)

     cAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCt                                                                      <  2:754062/81‑1 (MQ=255)

      aaaTCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCtt                                                                     <  2:754063/81‑1 (MQ=255)

       aaTCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTc                                                                    <  2:754064/81‑1 (MQ=255)

        aTCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTcc                                                                   <  1:754066/81‑1 (MQ=255)

        aTCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTcc                                                                   >  1:754100/1‑81 (MQ=255)

        aTCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTcc                                                                   <  1:754065/81‑1 (MQ=255)

             ccAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGtt                                                               >  1:754104/1‑80 (MQ=255)

                  aGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCg                                                          >  1:754099/1‑80 (MQ=255)

                     tGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAg                                                      >  2:754108/1‑81 (MQ=255)

                     tGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAg                                                      >  2:754103/1‑81 (MQ=255)

                         cAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTAt                                                  >  1:754110/1‑81 (MQ=255)

                           aatgtaatgtAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGa                                                <  1:754067/81‑1 (MQ=255)

                             tgtaatgtaaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAgg                                              >  2:754109/1‑81 (MQ=255)

                                aatgtaaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCg                                            >  2:754106/1‑80 (MQ=255)

                                    taaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATc                                        <  1:754069/80‑1 (MQ=255)

                                    taaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATc                                        <  1:754068/80‑1 (MQ=255)

                                             aTTTGCGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTg                               >  2:754117/1‑80 (MQ=255)

                                                  cGACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTg                          <  1:754070/80‑1 (MQ=255)

                                                   gACGCGTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGat                        <  1:754071/81‑1 (MQ=255)

                                                    aCGCGTCTCACAAGACGCTGTTTTGCTGCTTGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGata                       <  1:754072/81‑1 (MQ=255)

                                                     cgcgTCTCACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGatat                      >  2:754113/1‑81 (MQ=255)

                                                           tcACAAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                 >  2:754112/1‑80 (MQ=255)

                                                             acaAGACGCTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                 <  2:754073/78‑1 (MQ=255)

                                                                     cTGTTTTGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTaa      <  1:754074/81‑1 (MQ=255)

                                                                          ttGCTGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATgg  <  2:754075/80‑1 (MQ=255)

TGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGG  >  NC_000913/4142283‑4142436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`