Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,144,736	T→A	C248S (TGC→AGC)	pflD →	putative formate acetyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,144,736	0	T	A	100.0%	102.6 / NA	30	C248S (TGC→AGC)	pflD	putative formate acetyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/17);  total (13/17)

CGTCGCGAAGAGCTGCTGACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTC  >  NC_000913/4144658‑4144810                                                                               |                                                                           cGTCGCGAAGAGCTGCTGACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGc                                                                          <  2:754543/81‑1 (MQ=255)  gTCGCGAAGAGCTGCTGACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGcc                                                                         <  1:754544/81‑1 (MQ=255)     gcgAAGAGCTGCGGACTATTGCAGGGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAg                                                                       <  2:754545/80‑1 (MQ=255)       gAAGAGCTGCTGACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTa                                                                    >  2:754608/1‑81 (MQ=255)         agagCTGCTGACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTAtt                                                                  <  1:754546/81‑1 (MQ=255)         agagCTGCTGACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTAtt                                                                  >  2:754585/1‑81 (MQ=255)           agCTGCTGACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCt                                                                <  2:754547/81‑1 (MQ=255)             ctgctgACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTgg                                                              <  1:754548/81‑1 (MQ=255)                ctgACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTa                                                            >  2:754576/1‑80 (MQ=255)                ctgACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTAc                                                           <  2:754549/81‑1 (MQ=255)                    cTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATg                                                        >  2:754595/1‑80 (MQ=255)                        tGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAAcat                                                   >  2:754589/1‑81 (MQ=255)                         gCAGAGAACTCCCGCCATAACGCGAAACATAAGCCGCAGACGTTCTGGCAGGCGAGCAAGTTATACTGGTACATGAAcat                                                   >  2:754600/1‑80 (MQ=255)                          cAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAAcatc                                                  <  1:754550/80‑1 (MQ=255)                                    ccGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGc                                           <  1:754551/77‑1 (MQ=255)                                     cGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATAc                                      >  2:754594/1‑81 (MQ=255)                                            aCGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATcc                                >  2:754598/1‑80 (MQ=255)                                            aCGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATcc                                >  2:754597/1‑80 (MQ=255)                                                 cAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGcc                          <  1:754553/81‑1 (MQ=255)                                                 cAACATAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGcc                          <  1:754552/81‑1 (MQ=255)                                                      tAAGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTc                     <  2:754554/81‑1 (MQ=255)                                                        aGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCg                    <  2:754555/80‑1 (MQ=255)                                                        aGCCGCAGACGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGc                   <  1:754556/81‑1 (MQ=255)                                                                 cGTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTgg          >  2:754617/1‑81 (MQ=255)                                                                  gTTCTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTggg         >  1:754614/1‑81 (MQ=255)                                                                     cTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGgcg      >  2:754603/1‑81 (MQ=255)                                                                     cTGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGgc       <  1:754557/80‑1 (MQ=255)                                                                      tGGCAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGgcgc     >  2:754605/1‑81 (MQ=255)                                                                         cAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTc  <  1:754558/81‑1 (MQ=255)                                                                         cAGGCGAGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTc  <  1:754559/81‑1 (MQ=255)                                                                               |                                                                           CGTCGCGAAGAGCTGCTGACTATTGCAGAGATCTCCCGCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTC  >  NC_000913/4144658‑4144810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑