Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,144,842	C→T	S283L (TCA→TTA)	pflD →	putative formate acetyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,144,842	0	C	T	100.0%	124.0 / NA	36	S283L (TCA→TTA)	pflD	putative formate acetyltransferase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/21);  total (15/21)

CTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACAT  >  NC_000913/4144766‑4144914                                                                             |                                                                         cTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACattatt                                                                       >  1:754610/1‑80 (MQ=255) cTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACattatt                                                                       <  1:754569/80‑1 (MQ=255)  tGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTaa                                                                     >  2:754611/1‑81 (MQ=255)   gCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAAc                                                                    >  1:754615/1‑81 (MQ=255)    cAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTaa                                                                     <  2:754571/79‑1 (MQ=255)    cAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAAc                                                                    <  1:754570/80‑1 (MQ=255)      aTACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAAcc                                                                   >  1:754626/1‑79 (MQ=255)      aTACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCa                                                                 <  1:754573/81‑1 (MQ=255)      aTACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCa                                                                 <  2:754572/81‑1 (MQ=255)       tACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAg                                                                >  2:754625/1‑81 (MQ=255)       tACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAg                                                                <  1:754574/81‑1 (MQ=255)         cGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAggg                                                              <  2:754575/81‑1 (MQ=255)              ccAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCg                                                            >  2:754613/1‑78 (MQ=255)                aaCGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGAt                                                       <  1:754576/81‑1 (MQ=255)                  cGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATgc                                                     <  1:754577/81‑1 (MQ=255)                      aGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATgcggcg                                                 >  2:754618/1‑81 (MQ=255)                        ttCGCTATCGTTGGGGCCCCTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGtt                                               <  2:754578/81‑1 (MQ=255)                         tCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTc                                              >  1:754619/1‑81 (MQ=255)                         tCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTc                                              >  1:754627/1‑81 (MQ=255)                               tCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGaa                                         <  2:754579/80‑1 (MQ=255)                                 gTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAg                                       <  2:754580/80‑1 (MQ=255)                                    ggggCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAAc                                    <  2:754581/80‑1 (MQ=255)                                     gggCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTg                                  >  1:754612/1‑81 (MQ=255)                                         gcTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCg                              <  2:754582/81‑1 (MQ=255)                                          cTTCGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCg                              >  1:754622/1‑80 (MQ=255)                                             cGACCAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATc                          >  2:754628/1‑81 (MQ=255)                                                 cAGTATATGTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTa                      <  2:754583/81‑1 (MQ=255)                                                         gTTGCCGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGa               >  2:754620/1‑80 (MQ=255)                                                             ccGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGc          <  1:754585/81‑1 (MQ=255)                                                             ccGTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGc          <  1:754584/81‑1 (MQ=255)                                                               gTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCaa        >  1:754647/1‑81 (MQ=255)                                                               gTTCTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCaa        >  1:754643/1‑81 (MQ=255)                                                                  cTATCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAAATGCTCGAATCTTTATGGGTGAAATGCAACGa     >  2:754624/1‑81 (MQ=255)                                                                     tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGa     <  1:754589/78‑1 (MQ=255)                                                                     tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGAc    <  1:754587/79‑1 (MQ=255)                                                                     tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACa   <  1:754590/80‑1 (MQ=255)                                                                     tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACa   <  1:754586/80‑1 (MQ=255)                                                                     tCAGACATTATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACAt  <  2:754588/81‑1 (MQ=255)                                                                             |                                                                         CTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGCGAAGATGCGGCGTTCCTGAAAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACAT  >  NC_000913/4144766‑4144914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑