Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,086	G→A	S25N (AGC→AAC)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,086	0	G	A	100.0%	89.4 / NA	26	S25N (AGC→AAC)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/13);  total (13/13)

GCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGAT  >  NC_000913/4156010‑4156159                                                                             |                                                                          gCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCa                                                                       >  1:757127/1‑81 (MQ=255)   aaTGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACt                                                                     >  2:757126/1‑81 (MQ=255)       atgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTg                                                                 <  2:757092/81‑1 (MQ=255)       atgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTg                                                                 <  2:757091/81‑1 (MQ=255)        tgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGa                                                                <  1:757093/81‑1 (MQ=255)           aTCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAAtt                                                             <  2:757094/81‑1 (MQ=255)              cATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATc                                                          <  2:757095/81‑1 (MQ=255)               attaattaTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCg                                                         <  1:757096/81‑1 (MQ=255)                   attaTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGa                                                      <  2:757097/80‑1 (MQ=255)                     taTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGt                                                    >  1:757139/1‑80 (MQ=255)                          aaCTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATc                                              <  2:757098/81‑1 (MQ=255)                           aCTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCa                                             <  2:757099/81‑1 (MQ=255)                             tGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGc                                           >  1:757131/1‑81 (MQ=255)                                     gTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTg                                   <  2:757100/81‑1 (MQ=255)                                        ctgctgGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGt                                 >  1:757149/1‑80 (MQ=255)                                          gctgGATAGTGAAGAGGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAt                              >  1:757146/1‑81 (MQ=255)                                                      agagGCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggcgg                  >  2:757134/1‑81 (MQ=255)                                                         ggCGCTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCAcggcggcgg               >  1:757143/1‑81 (MQ=255)                                                           cgcTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGtt             >  1:757147/1‑81 (MQ=255)                                                            gcTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTg            >  1:757136/1‑81 (MQ=255)                                                             cTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGc           <  1:757101/81‑1 (MQ=255)                                                             cTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGc           >  1:757150/1‑81 (MQ=255)                                                             cTGGAACGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGc           >  2:757140/1‑81 (MQ=255)                                                                   cGTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCgtggtg     >  2:757141/1‑81 (MQ=255)                                                                    gTCTGTTTAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGtggtgg    <  2:757102/81‑1 (MQ=255)                                                                         tttAACGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGAt  <  2:757103/78‑1 (MQ=255)                                                                             |                                                                          GCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGCACGGCGGCGGTTGCGTGGTGGAT  >  NC_000913/4156010‑4156159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑