Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,820	C→T	H526Y (CAC→TAC)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,820	0	C	T	100.0%	164.2 / NA	46	H526Y (CAC→TAC)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/24);  total (22/24)

CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAC  >  NC_000913/4182743‑4182897                                                                              |                                                                              cagcagTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtac                                                                             >  2:762663/1‑80 (MQ=255)  agcagTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGtaca                                                                            >  2:762679/1‑80 (MQ=255)  agcagTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACaa                                                                           <  1:762608/81‑1 (MQ=255)   gcagTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACaaa                                                                          <  1:762609/81‑1 (MQ=255)     agTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcg                                                                        >  2:762683/1‑81 (MQ=255)      gTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcgt                                                                       >  2:762681/1‑81 (MQ=255)        gAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcgtcg                                                                     >  1:762670/1‑81 (MQ=255)         aaaGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAAcgtcg                                                                     <  2:762610/80‑1 (MQ=255)             agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTAtct                                                                >  2:762700/1‑81 (MQ=255)             agTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTAtct                                                                <  1:762611/81‑1 (MQ=255)                 cttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCg                                                             <  1:762612/80‑1 (MQ=255)                 cttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCg                                                             <  2:762613/80‑1 (MQ=255)                   tcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCAc                                                          >  1:762695/1‑81 (MQ=255)                    cGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGc                                                            <  1:762614/78‑1 (MQ=255)                    cGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACt                                                         >  2:762674/1‑81 (MQ=255)                     ggTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACt                                                         <  2:762615/80‑1 (MQ=255)                     ggTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTc                                                        >  1:762675/1‑81 (MQ=255)                     ggTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTc                                                        >  2:762678/1‑81 (MQ=255)                        tCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGc                                                     >  2:762686/1‑81 (MQ=255)                          cagccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGcc                                                    <  1:762616/80‑1 (MQ=255)                            gccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAg                                                 <  2:762617/81‑1 (MQ=255)                              cagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGc                                               >  1:762669/1‑81 (MQ=255)                              cagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGc                                               >  2:762689/1‑81 (MQ=255)                                gcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCgg                                             >  1:762676/1‑81 (MQ=255)                                   gTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCt                                          >  2:762690/1‑81 (MQ=255)                                   gTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCt                                          >  2:762693/1‑81 (MQ=255)                                   gTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCt                                          >  2:762687/1‑81 (MQ=255)                                    tctcAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCt                                          <  2:762618/80‑1 (MQ=255)                                          tttATGGACCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGt                                   <  2:762619/81‑1 (MQ=255)                                                 aCCAGAACAACCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTg                            >  1:762697/1‑81 (MQ=255)                                                        caacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCtt                     <  1:762620/81‑1 (MQ=255)                                                         aacCCGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTc                    <  2:762621/81‑1 (MQ=255)                                                           cccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCg                   >  2:762677/1‑80 (MQ=255)                                                           cccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa                  <  2:762622/81‑1 (MQ=255)                                                           cccGCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa                  <  2:762623/81‑1 (MQ=255)                                                              gCTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCTAAGt               >  1:762685/1‑81 (MQ=255)                                                               cTGTCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGtt              >  1:762698/1‑81 (MQ=255)                                                                  tCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTc             <  1:762626/79‑1 (MQ=255)                                                                  tCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCg            <  1:762624/80‑1 (MQ=255)                                                                  tCTGAGATTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCg            <  1:762625/80‑1 (MQ=255)                                                                      agaTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACg       <  2:762627/81‑1 (MQ=255)                                                                        aTTACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTa     <  2:762628/81‑1 (MQ=255)                                                                         ttACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTac    <  2:762630/81‑1 (MQ=255)                                                                         ttACGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTac    <  1:762629/81‑1 (MQ=255)                                                                           aCGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac  >  2:762684/1‑81 (MQ=255)                                                                           aCGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac  >  1:762705/1‑81 (MQ=255)                                                                           aCGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac  >  1:762666/1‑81 (MQ=255)                                                                           aCGTACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTaca   <  2:762631/80‑1 (MQ=255)                                                                              |                                                                              CAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAC  >  NC_000913/4182743‑4182897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑