Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,140	A→G	I966V (ATC→GTC)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,140	0	A	G	100.0%	106.8 / NA	30	I966V (ATC→GTC)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

AAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  NC_000913/4184064‑4184213                                                                             |                                                                          aaaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCt                                                                       <  1:762975/81‑1 (MQ=255)  aaaGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTc                                                                      <  1:762976/81‑1 (MQ=255)    aGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGa                                                                    <  1:762977/81‑1 (MQ=255)        aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCg                                                                >  1:763022/1‑81 (MQ=255)              gcgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTg                                                          >  1:763012/1‑81 (MQ=255)               cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGt                                                         <  2:762978/81‑1 (MQ=255)               cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGt                                                         >  2:763024/1‑81 (MQ=255)                gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGtt                                                        <  1:762979/81‑1 (MQ=255)                 cTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTc                                                       <  1:762980/81‑1 (MQ=255)                      aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCg                                                  <  2:762981/81‑1 (MQ=255)                        tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTa                                                <  1:762982/81‑1 (MQ=255)                             gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgt                                           >  2:763020/1‑81 (MQ=255)                              aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtg                                          >  1:763042/1‑81 (MQ=255)                               aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc                                         <  2:762983/81‑1 (MQ=255)                               aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc                                         >  2:763025/1‑81 (MQ=255)                               aaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgc                                         <  1:762984/81‑1 (MQ=255)                                    aGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc                                    <  1:762985/81‑1 (MQ=255)                                     gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct                                   >  2:763015/1‑81 (MQ=255)                                     gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgct                                   <  2:762986/81‑1 (MQ=255)                                         aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt                                <  1:762987/80‑1 (MQ=255)                                              ggCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          >  1:763033/1‑81 (MQ=255)                                               gCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGt                         >  2:763038/1‑81 (MQ=255)                                               gCGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGt                         <  2:762988/81‑1 (MQ=255)                                                cGAAGAAAGACCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        >  1:763041/1‑81 (MQ=255)                                                   agaaagaCCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg                     >  1:763018/1‑81 (MQ=255)                                                         aCCTGTCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  1:763029/1‑81 (MQ=255)                                                              tCTGAAGAACTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          <  1:762989/81‑1 (MQ=255)                                                                   agaaCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     <  1:762990/81‑1 (MQ=255)                                                                    gaaCTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc    >  1:763027/1‑81 (MQ=255)                                                                       cTGCAGGTCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  1:763046/1‑80 (MQ=255)                                                                             |                                                                          AAAAGACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  NC_000913/4184064‑4184213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑