Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,319,653	G→A	F337F (TTC→TTT)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,319,653	0	G	A	100.0%	132.9 / NA	38	F337F (TTC→TTT)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/25);  total (13/25)

GCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGGAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACG  >  NC_000913/4319578‑4319728                                                                            |                                                                            gCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATc                                                                        <  2:792264/81‑1 (MQ=255)    aaGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGAc                                                                     <  1:792266/81‑1 (MQ=255)    aaGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGAc                                                                     <  1:792265/81‑1 (MQ=255)       ttGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACg                                                                    >  1:792318/1‑79 (MQ=255)        tGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAg                                                                 >  2:792327/1‑81 (MQ=255)           aGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTgg                                                              >  2:792334/1‑81 (MQ=255)             ccGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTgggg                                                            <  2:792267/81‑1 (MQ=255)              cGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTggggg                                                           <  2:792268/81‑1 (MQ=255)                   aGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGttt                                                      <  1:792269/81‑1 (MQ=255)                   aGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGtt                                                       <  1:792270/80‑1 (MQ=255)                    gATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTGGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTg                                                     <  1:792271/81‑1 (MQ=255)                     aTTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGa                                                    <  2:792272/81‑1 (MQ=255)                         gCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgtg                                                >  2:792337/1‑81 (MQ=255)                             tGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGgtgt                                               >  2:792326/1‑78 (MQ=255)                             tGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTgag                                            <  2:792273/81‑1 (MQ=255)                                  cTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACaa                                        <  1:792274/80‑1 (MQ=255)                                    gCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGc                                     >  2:792309/1‑81 (MQ=255)                                        cTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGc                                 >  2:792333/1‑81 (MQ=255)                                           ttGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGc                              <  2:792275/81‑1 (MQ=255)                                             gCAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCt                             <  2:792276/80‑1 (MQ=255)                                              cAGACGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTc                           <  2:792277/81‑1 (MQ=255)                                                 aCGTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGAc                        <  1:792278/81‑1 (MQ=255)                                                   gTTTGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGt                      >  2:792348/1‑81 (MQ=255)                                                    tttGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGGAGTGTGAAACAAAGCCTGCGGCTTCGACGtt                     <  2:792280/81‑1 (MQ=255)                                                    tttGAGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGtt                     <  2:792279/81‑1 (MQ=255)                                                        aGTAGCTCCAGTTCGGCCTGAAAATCGACGTGGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCg                 <  2:792283/81‑1 (MQ=255)                                                        aGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTc                  <  2:792284/80‑1 (MQ=255)                                                        aGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTc                  <  1:792282/80‑1 (MQ=255)                                                        aGTAGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCg                 <  1:792281/81‑1 (MQ=255)                                                           aGCTCCAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCa              >  2:792335/1‑81 (MQ=255)                                                               ccAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCAtgtg          >  2:792344/1‑81 (MQ=255)                                                               ccAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCAtgtg          >  1:792341/1‑81 (MQ=255)                                                                cAGTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCAtgt           <  1:792285/79‑1 (MQ=255)                                                                  gTTCGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGc         >  1:792336/1‑79 (MQ=255)                                                                     cGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGc     <  2:792287/80‑1 (MQ=255)                                                                     cGGCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGc     <  1:792286/80‑1 (MQ=255)                                                                       gCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCAc   >  2:792358/1‑80 (MQ=255)                                                                       gCCTGAAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACg  <  1:792288/81‑1 (MQ=255)                                                                            |                                                                            GCAAAGTTGTAGCCGCTCAGATTAGCCATGGTTCTGCTCCTGTTGCAGACGTTTGAGTAGCTCCAGTTCGGCCTGGAAATCGACGTAGTGGGGGAGTTTGAGGTGTGAGACAAAGCCTGCGGCTTCGACGTTGTCGGCATGTGCCAGCACG  >  NC_000913/4319578‑4319728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑