Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	447,488	T→C	N73S (AAT→AGT)	cyoE ←	heme O synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	447,488	0	T	C	100.0%	81.1 / NA	24	N73S (AAT→AGT)	cyoE	heme O synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/13);  total (11/13)

AAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAAC  >  NC_000913/447413‑447561                                                                            |                                                                          aaGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGActcttc                                                                      <  1:86613/81‑1 (MQ=255)    ccAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTc                                                                   >  2:86670/1‑81 (MQ=255)     cAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTcc                                                                  <  2:86614/81‑1 (MQ=255)      aGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCt                                                                 <  2:86615/81‑1 (MQ=255)       gCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCtt                                                                >  1:86657/1‑81 (MQ=255)             cccAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCAt                                                           <  2:86616/80‑1 (MQ=255)               cAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCtt                                                        >  2:86661/1‑81 (MQ=255)                aGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCttt                                                       <  1:86617/81‑1 (MQ=255)                aGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCttt                                                       >  1:86653/1‑81 (MQ=255)                 gCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTc                                                      <  1:86618/81‑1 (MQ=255)                          cGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGatat                                             >  2:86645/1‑81 (MQ=255)                           gTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATc                                            >  2:86654/1‑81 (MQ=255)                                ccAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGt                                       <  2:86619/81‑1 (MQ=255)                                  aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTc                                      >  1:86647/1‑80 (MQ=255)                                  aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTc                                      >  2:86655/1‑80 (MQ=255)                                      ggaCAGCAGGAGAGATCAGGCCTTCCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATg                                  >  2:86658/2‑80 (MQ=255)                                                     tCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAacacac                  <  1:86620/81‑1 (MQ=255)                                                     tCAGGCCTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAacaca                   >  2:86659/1‑80 (MQ=255)                                                           cTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCg            <  1:86622/81‑1 (MQ=255)                                                           cTTTCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCg            <  1:86621/81‑1 (MQ=255)                                                              tCACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGAc          <  1:86623/80‑1 (MQ=255)                                                               cACCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACg         <  1:86624/80‑1 (MQ=255)                                                                aCCAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGc        <  1:86625/80‑1 (MQ=255)                                                                 ccAGCACCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCca      <  2:86626/81‑1 (MQ=255)                                                                      aCCCGACTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAAc  >  2:86664/1‑80 (MQ=255)                                                                            |                                                                          AAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAACACACAACCCGACGCCACAAC  >  NC_000913/447413‑447561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑