Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,334,130	C→T	W487* (TGG→TGA)	eptA ←	phosphoethanolamine transferase EptA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,334,130	0	C	T	100.0%	67.9 / NA	20	W487* (TGG→TGA)	eptA	phosphoethanolamine transferase EptA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/15);  total (5/15)

AATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGCCAT  >  NC_000913/4334054‑4334203                                                                             |                                                                          aaTAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTcac                                                                        <  1:795628/80‑1 (MQ=255)   tAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCAcagc                                                                     <  1:795629/81‑1 (MQ=255)      tgtTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACagcagc                                                                  <  2:795630/81‑1 (MQ=255)       gtTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAgcagca                                                                 >  1:795635/1‑81 (MQ=255)        ttGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAgcagca                                                                 <  1:795631/80‑1 (MQ=255)            gTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGc                                                            <  2:795632/81‑1 (MQ=255)                 cgcCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCt                                                        <  2:795633/80‑1 (MQ=255)                       tttttGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGtttt                                                   <  2:795634/79‑1 (MQ=255)                         tttGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTgg                                                <  2:795635/80‑1 (MQ=255)                             caggcagTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATc                                           <  2:795636/81‑1 (MQ=255)                              aggcagTTCTGGTCAACCTGATACCATTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTGGCCTATcc                                          >  2:795655/1‑81 (MQ=255)                               ggcagTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCg                                         <  1:795637/81‑1 (MQ=255)                                  agTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCgggg                                      >  1:795650/1‑81 (MQ=255)                                   gTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGc                                     <  1:795639/81‑1 (MQ=255)                                   gTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGc                                     <  2:795638/81‑1 (MQ=255)                                                  aTACCGTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAg                      <  2:795640/81‑1 (MQ=255)                                                       gTTTTTGATAATCCTCCGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGt                 <  1:795641/81‑1 (MQ=255)                                                                    ctccGACAGTCACAGCAGCATCTGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGcc    <  2:795642/81‑1 (MQ=255)                                                                      ccGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGCCAt  >  1:795659/1‑81 (MQ=255)                                                                      ccGACAGTCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGCCAt  >  2:795660/1‑81 (MQ=255)                                                                             |                                                                          AATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGCAGATAGATGCCAT  >  NC_000913/4334054‑4334203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑