Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,347,445	C→T	V434I (GTC→ATC)	dcuB ←	anaerobic C4‑dicarboxylate transporter DcuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,347,445	0	C	T	100.0%	134.0 / NA	38	V434I (GTC→ATC)	dcuB	anaerobic C4‑dicarboxylate transporter DcuB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/12);  total (26/12)

GTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGC  >  NC_000913/4347369‑4347522                                                                             |                                                                              gTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCAc                                                                           >  1:798267/1‑81 (MQ=255)    ccGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGa                                                                         >  1:798259/1‑80 (MQ=255)    ccGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGAt                                                                        <  1:798229/81‑1 (MQ=255)    ccGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGAt                                                                        <  2:798230/81‑1 (MQ=255)      gcgTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGACGATGCACGATAc                                                                      >  1:798256/1‑81 (MQ=255)      gcgTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATAc                                                                      >  2:798257/1‑81 (MQ=255)      gcgTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATAc                                                                      <  2:798231/81‑1 (MQ=255)          aGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCt                                                                   >  1:798255/1‑80 (MQ=255)           gTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTc                                                                  <  2:798232/80‑1 (MQ=255)                gggCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACAcc                                                             >  2:798280/1‑80 (MQ=255)                  gcggcACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAAt                                                          >  1:798263/1‑81 (MQ=255)                     gcACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCaa                                                       >  1:798260/1‑81 (MQ=255)                       aCGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAAcc                                                     >  1:798269/1‑81 (MQ=255)                          cAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCg                                                  >  2:798264/1‑81 (MQ=255)                           aaaGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCgg                                                 >  1:798261/1‑81 (MQ=255)                             aGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCgg                                                 >  2:798283/1‑79 (MQ=255)                             aGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCa                                               <  2:798233/81‑1 (MQ=255)                                   tttATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATaa                                         <  1:798234/81‑1 (MQ=255)                                   tttATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATaa                                         <  1:798235/81‑1 (MQ=255)                                         aGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtgt                                   >  1:798285/1‑81 (MQ=255)                                         aGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCtgt                                   >  1:798294/1‑81 (MQ=255)                                              ccGTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtg                              <  1:798236/81‑1 (MQ=255)                                                gTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgat                            >  1:798278/1‑81 (MQ=255)                                                gTACATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgat                            <  2:798237/81‑1 (MQ=255)                                                  aCATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgatga                          >  2:798271/1‑81 (MQ=255)                                                  aCATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTtgatga                          >  1:798286/1‑81 (MQ=255)                                                   cATCGCGGCGAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGAc                         <  1:798238/81‑1 (MQ=255)                                                         ggcgAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGc                    >  2:798276/1‑80 (MQ=255)                                                         ggcgAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGc                    >  2:798300/1‑80 (MQ=255)                                                           cgAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAc                 <  2:798239/81‑1 (MQ=255)                                                           cgAAGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAc                 >  2:798281/1‑81 (MQ=255)                                                              aGATCCAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGa              <  1:798240/81‑1 (MQ=255)                                                                  ccAGCCGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGAtgtg          >  2:798284/1‑81 (MQ=255)                                                                       cGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATgtgggtgg     >  1:798265/1‑81 (MQ=255)                                                                       cGAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATgtgggtgg     >  2:798277/1‑81 (MQ=255)                                                                        gAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGggtggt    >  1:798272/1‑81 (MQ=255)                                                                        gAAGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGggtggt    >  2:798291/1‑81 (MQ=255)                                                                          aGATGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGc  >  1:798279/1‑81 (MQ=255)                                                                             |                                                                              GTGCCGCGTAGTCACGGGCGGCACGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGC  >  NC_000913/4347369‑4347522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑